Chicken Bone morphogenetic protein 2(BMP2) ELISA kit

Code CSB-EL002736CH
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q90751
Lead Time 5-7 working days
Abbreviation BMP2
Protein Biological Process 1 Developmental Protein
Target Name bone morphogenetic protein 2
Alias BMP2A,
Species Gallus gallus (Chicken)
Protein Biological Process 3 Chondrogenesis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken BMP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 89-103
1:2 Average % 95
Range % 85-102
1:4 Average % 95
Range % 89-99
1:8 Average % 95
Range % 90-105
Recovery
The recovery of chicken BMP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 87-99
EDTA plasma (n=4) 88 84-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 1.972 1.998 1.985 1.864
1000 1.641 1.625 1.633 1.512
500 1.205 1.173 1.189 1.068
250 0.799 0.782 0.791 0.670
125 0.551 0.545 0.548 0.427
62.5 0.356 0.347 0.352 0.231
31.25 0.257 0.252 0.255 0.134
0 0.118 0.123 0.121  
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Function Negatively regulates the structure and function of the limb apical ectodermal ridge.
Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

STRING: 9031.ENSGALP00000014334

UniGene: Gga.3950

Pathway Hippo signaling pathway
TGF-beta signaling pathway

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