Chicken Insulin-like growth factor II(IGF2) ELISA kit

Code CSB-EL011088CH
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P33717
Lead Time 7-14 working days
Abbreviation IGF2
Target Name insulin-like growth factor 2 (somatomedin A)
Alias C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, OTTHUMP00000011018|OTTHUMP00000011157|insulin-like growth factor 2|insulin-like growth factor II|insulin-like growth factor type 2|putative insulin-like
Species Gallus gallus (Chicken)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Target Details This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3 region and to the upstream INS gene at the 5 region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene.
HGNC 5466
RGD 2870
MGI 96434
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 107
Range % 103-110
1:200 Average % 86
Range % 83-90
1:400 Average % 97
Range % 94-101
1:800 Average % 93
Range % 90-96
Recovery
The recovery of chicken IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-92
EDTA plasma (n=4) 84 80-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average
4000 0.096 0.099 0.098
2000 0.201 0.210 0.206
1000 0.336 0.360 0.348
500 0.501 0.531 0.516
250 0.763 0.751 0.757
125 1.003 1.065 1.034
62.5 1.320 1.400 1.360
0 2.137 2.200 2.169
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Function The insulin-like growth factors possess growth-promoting activity.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: gga:395097

STRING: 9031.ENSGALP00000010570

UniGene: Gga.8511

Pathway MAPK signaling pathway
PI3K-Akt signaling pathway
Ras signaling pathway

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