Chicken Interferon α,IFN-α ELISA Kit

Code CSB-E10071Ch
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Interferon α,IFN-α
Alternative Names
IFNA1; ELISA Kit; IFNA2 ELISA Kit; Interferon type A1/A2 ELISA Kit
Abbreviation
IFNA1
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %83
Range %80-95
1:2Average %95
Range %91-101
1:4Average %93
Range %86-98
1:8Average %95
Range %82-98
Recovery
The recovery of chicken IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)9692-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2001.726 1.745 1.736 1.631
1001.336 1.327 1.332 1.227
500.957 0.976 0.967 0.862
250.621 0.631 0.626 0.521
12.50.437 0.409 0.423 0.318
6.250.303 0.301 0.302 0.197
3.120.234 0.244 0.239 0.134
00.107 0.102 0.105  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Chicken IFNA1 ELISA Kit was designed for the quantitative measurement of Chicken IFNA1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

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Target Background

Function
(From Uniprot)
Has antiviral activities.
Subcellular Location
Secreted.
Protein Families
Alpha/beta interferon family
Database Links
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