Chicken Interferon γ,IFN-γ ELISA Kit

Code CSB-E08550Ch
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interferon, gamma
Alternative Names
IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
Abbreviation
IFNG
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.125 pg/mL-800 pg/mL
Sensitivity
3.125 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 91-100  
1:2 Average % 92  
Range % 88-97  
1:4 Average % 102  
Range % 98-106  
1:8 Average % 94  
Range % 90-98  
Recovery
The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 93-101  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
800 0.178 0.182 0.180    
200 0.245 0.233 0.239    
50 0.457 0.482 0.470    
12.5 0.848 0.817 0.833    
3.125 1.396 1.364 1.380    
0 2.237 2.242 2.240    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Chicken IFNG ELISA Kit was designed for the quantitative measurement of Chicken IFNG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.125 pg/mL-800 pg/mL and the sensitivity is 3.125 pg/mL.

Citations

Customer Reviews and Q&A

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 Q&A
Q:

Do you know if the chicken IFN-gamma kit shows cross-reactivity with other species?

A:

There is no cross-reactivity with human, rat, and mouse recombinant proteins.

Target Background

Function
(From Uniprot)
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Gene References into Functions
  1. Knockdown of IFN-gamma did not seem to fully abrogate vaccine-induced protection against Marek's disease in chickens. PMID: 25673902
  2. Vitiligo in Smyth line chickens appears to be a Th1-polarized autoimmune disease, whereby IFN-gamma expression is strongly associated with parallel increases in IL-10 and IL-21, particularly during the early and active stages of vitiligo. PMID: 22113479
  3. The dose-response effects of supplemental dietary chormium on interferon-gamma expression in response to Newcastle disease virus vaccine in broiler chickens is reported. PMID: 17888472
  4. The pivotal role of chicken interferon-gamma in the pathogenesis and immunosuppression of infectious bursal disease virus infection in in vivo, ex vivo and in vitro experiments are reported. PMID: 17899460
  5. that in vitro stimulation with IBV resulted in ChIFN-gamma production in splenocytes of both infected birds and uninfected birds PMID: 19070879

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Subcellular Location
Secreted.
Protein Families
Type II (or gamma) interferon family
Database Links
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