Chicken Lipopolysaccharide-induced tumor necrosis factor-alpha factor(LITAF) ELISA kit

Instructions
Code CSB-EL012985CH
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name lipopolysaccharide-induced TNF factor
Alternative Names LITAF ELISA Kit; SIMPLE ELISA Kit; Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog ELISA Kit; LPS-induced TNF-alpha factor homolog ELISA Kit; Small integral membrane protein of lysosome/late endosome ELISA Kit
Abbreviation LITAF
Uniprot No. Q8QGW7
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken LITAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 85-95
1:2 Average % 104
Range % 100-108
1:4 Average % 95
Range % 90-100
1:8 Average % 84
Range % 80-88
Recovery
The recovery of chicken LITAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 92-101
EDTA plasma (n=4) 89 85-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.105 2.003 2.054 1.949
2000 1.812 1.716 1.764 1.659
1000 1.478 1.487 1.483 1.378
500 1.196 1.159 1.178 1.073
250 0.709 0.720 0.715 0.610
125 0.483 0.475 0.479 0.374
62.5 0.274 0.281 0.278 0.173
0 0.107 0.102 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role in the nucleus in regulating the expression of numerous cytokines.
Gene References into Functions
  1. The results showed that Se deficiency induced high expression levels of PTGE, COX-2, TNF-alpha, and NF-kappaB in the gastrointestinal tract tissues. PMID: 26899319
  2. The results showed that the expression levels of IL-6, IL-8, TNF-alpha, and NF-kappaB which seemed to be a critical mediator in the inflammatory response tended to increase in the birds chronically treated with As2O3. However, the mRNA expression levels of IL-4, IL-12beta, and IL-1beta were decreased in the experiment. PMID: 26276563
  3. Data show that IL-10, IFN-gamma and TNF-alpha contents in the cecal tonsil were significantly decreased in the 30, 45 and 60 mg/kg groups, while IL-6 content in the cecal tonsil was only decreased in 60 mg/kg at 14 and 28 days of age. PMID: 22270623
  4. The expression of interleukin(IL)-1 beta, IL-6, and tumor necrosis factor (TNF)-alpha in amyoidosis arthropathy in chickens was studied. PMID: 18612836
  5. IL1B and LITAF mRNAs are increased in the vagina during insemination. This response may lead to sperm degradation and elimination. PMID: 18539728

Show More

Hide All

Subcellular Location Cytoplasm, Nucleus, Lysosome membrane, Peripheral membrane protein, Cytoplasmic side, Early endosome membrane, Late endosome membrane, Endosome membrane, Peripheral membrane protein, Cytoplasmic side, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Golgi apparatus membrane
Protein Families CDIP1/LITAF family
Database Links

KEGG: gga:374125

STRING: 9031.ENSGALP00000005086

UniGene: Gga.3383

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1