Dog Apolipoprotein B (APOB) ELISA kit

Instructions
Code CSB-EL001918DO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein B (including Ag(x) antigen) (APOB)
Alternative Names FLDB, LDLCQ4, apoB-100|apoB-48|apolipoprotein B|apolipoprotein B48
Abbreviation APOB
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 ng/mL-6000 ng/mL
Sensitivity 23.44 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog AOPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 93
Range % 85-100
1:400 Average % 96
Range % 89-99
1:800 Average % 95
Range % 91-102
1:1600 Average % 95
Range % 89-100
Recovery
The recovery of dog AOPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-96
EDTA plasma (n=4) 93 87-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average  
6000 0.091 0.096 0.094  
3000 0.145 0.152 0.149  
1500 0.203 0.209 0.206  
750 0.332 0.326 0.329  
375 0.489 0.494 0.492  
187.5 0.637 0.605 0.621  
93.75 0.885 0.837 0.861  
0 1.576 1.568 1.572  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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