Dog Apolipoprotein B (APOB) ELISA kit

Code CSB-EL001918DO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Protein Biological Process 2 Lipogenesis and lipometabolism
Abbreviation APOB
Protein Biological Process 1 Apolipoprotein
Target Name apolipoprotein B (including Ag(x) antigen) (APOB)
Alias FLDB, LDLCQ4, apoB-100|apoB-48|apolipoprotein B|apolipoprotein B48
Species Canis lupus familiaris (Dog) (Canis familiaris)
Protein Biological Process 3 Cholesterol metabolism
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 ng/mL-6000 ng/mL
Sensitivity 23.44 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
HGNC 603
RGD 2129
MGI 88052
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog AOPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 93
Range % 85-100
1:400 Average % 96
Range % 89-99
1:800 Average % 95
Range % 91-102
1:1600 Average % 95
Range % 89-100
Recovery
The recovery of dog AOPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-96
EDTA plasma (n=4) 93 87-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average  
6000 0.091 0.096 0.094  
3000 0.145 0.152 0.149  
1500 0.203 0.209 0.206  
750 0.332 0.326 0.329  
375 0.489 0.494 0.492  
187.5 0.637 0.605 0.621  
93.75 0.885 0.837 0.861  
0 1.576 1.568 1.572  
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