Dog Brain-derived neurotrophic factor(BDNF) ELISA kit

Code CSB-EL002655DO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q7YRB4
Lead Time 7-14 working days
Abbreviation BDNF
Target Name brain-derived neurotrophic factor
Alias MGC34632, neurotrophin
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Target Details This protein is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer s and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene.
HGNC 1033
RGD 2202
MGI 88145
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 81-92
1:2 Average % 97
Range % 92-104
1:4 Average % 92
Range % 85-96
1:8 Average % 101
Range % 98-105
Recovery
The recovery of dog BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 82-93
EDTA plasma (n=4) 93 86-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.123 2.005 2.064 1.962
100 1.765 1.675 1.720 1.618
50 1.298 1.258 1.278 1.176
25 0.913 0.909 0.911 0.809
12.5 0.623 0.600 0.612 0.510
6.25 0.415 0.407 0.411 0.309
3.12 0.259 0.256 0.258 0.156
0 0.103 0.101 0.102  
References
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Function During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS (By similarity).
Subcellular Location Secreted
Protein Families NGF-beta family
Database Links

KEGG: cfa:403461

STRING: 9615.ENSCAFP00000014984

UniGene: Cfa.170

Pathway Ras signaling pathway
cAMP signaling pathway
MAPK signaling pathway
PI3K-Akt signaling pathway
Inflammatory Pain
Neurotrophin signaling pathway

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