Dog Interleukin 1β (IL- 1β) ELISA Kit

Instructions
Code CSB-E13836c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 1, beta
Alternative Names IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
Abbreviation IL1B
Uniprot No. Q28292
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %83-100
1:2Average %94
Range %91-99
1:4Average %95
Range %90-105
1:8Average %94
Range %86-100
Recovery
The recovery of dog IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-102
EDTA plasma (n=4)9790-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.585 2.675 2.630 2.512
2502.319 2.412 2.366 2.248
1251.957 1.972 1.965 1.847
62.51.432 1.453 1.443 1.325
31.250.744 0.719 0.732 0.614
15.630.517 0.508 0.513 0.395
7.80.385 0.377 0.381 0.263
00.118 0.117 0.118  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families IL-1 family
Database Links

STRING: 9615.ENSCAFP00000010762

UniGene: Cfa.33592

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