Dog Interleukin-5(IL5) ELISA kit

Instructions
Code CSB-EL011662DO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 5 (colony-stimulating factor, eosinophil)
Alternative Names IL5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
Abbreviation IL5
Uniprot No. Q95J76
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 82-89
1:2 Average % 93
Range % 90-96
1:4 Average % 88
Range % 84-93
1:8 Average % 102
Range % 98-105
Recovery
The recovery of dog IL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 85-89
EDTA plasma (n=4) 91 87-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.869 2.901 2.885 2.791
100 2.374 2.304 2.339 2.245
50 1.620 1.638 1.629 1.535
25 0.931 0.905 0.918 0.824
12.5 0.434 0.441 0.438 0.344
6.25 0.239 0.246 0.243 0.149
3.12 0.141 0.146 0.144 0.050
0 0.095 0.092 0.094  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Subcellular Location Secreted
Protein Families IL-5 family
Database Links

KEGG: cfa:403790

STRING: 9615.ENSCAFP00000001224

UniGene: Cfa.3486

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