Dog Leptin(LEP) ELISA kit

Instructions
Code CSB-EL012870DO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name leptin
Alternative Names LEP ELISA Kit; OBLeptin ELISA Kit; Obesity factor ELISA Kit
Abbreviation LEP
Uniprot No. O02720
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum,plasma, tissue homogenates.
Detection Range 4.7 ng/ml -300 ng/ml
Sensitivity 1.175 ng/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (By similarity). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption (By similarity). Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression. Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells. Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (By similarity). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation (By similarity).
Gene References into Functions
  1. This is the first report regarding the pattern of leptin during whole pregnancy and lactation. The present work lays the groundwork for further studies on endocrine homeostatic mechanisms using the dog as a model for human medicine PMID: 28583599
  2. Leptin and insulin-like growth factor 1 may support spermatogenesis in adult dogs and mediate Leydig cell function PMID: 28101891
  3. This study evaluated the differences in leptin, adiponectin, and serotonin between lean and obese dogs. PMID: 24886049
  4. These results indicate that both leptin and leptin receptor mRNA are present in the canine corpus luteum during the non-pregnant luteal phase and pregnancy. PMID: 23279462
  5. fasting plasma leptin concentration was directly negatively associated with fasting insulin sensitivity and positively associated with beta-cell function PMID: 21474268
  6. high-fat feeding induces resistance to the coronary vascular effects of leptin, and this represents an early protective adaptation against endothelial dysfunction PMID: 15894577
  7. plasma leptin concentration analysis in dogs PMID: 16919691
  8. Leptin dilates canine small mesenteric arteries and veins by a mechanism involving endothelial release of nitric oxide. PMID: 17600555

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Subcellular Location Secreted
Protein Families Leptin family
Database Links

KEGG: cfa:403616

STRING: 9615.ENSCAFP00000002433

UniGene: Cfa.3476

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