Dog Metalloproteinase inhibitor 1(TIMP1) ELISA kit

Code CSB-EL023560DO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P81546
Lead Time 5-7 working days
Abbreviation TIMP1
Protein Biological Process 1 Immunity
Target Name TIMP metallopeptidase inhibitor 1
Alias RP1-230G1.3, CLGI, EPA, EPO, FLJ90373, HCI, TIMP, erythroid potentiating activity|fibroblast collagenase inhibitor|tissue inhibitor of metalloproteinase 1
Species Canis lupus familiaris (Dog) (Canis familiaris)
Protein Biological Process 3 Erythrocyte maturation
Sample Types serum, plasma, tissue homogenates
Detection Range 39 ng/mL-2500 ng/mL
Sensitivity 9.75 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
HGNC 11820
RGD 621675
MGI 98752
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog TIMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-91
1:2 Average % 90
Range % 85-95
1:4 Average % 97
Range % 92-110
1:8 Average % 104
Range % 100-108
Recovery
The recovery of dog TIMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-100
EDTA plasma (n=4) 92 88-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
2500 2.193 2.052 2.123 1.958
1250 1.444 1.513 1.479 1.314
625 0.898 0.854 0.876 0.711
312 0.486 0.509 0.498 0.333
156 0.358 0.364 0.361 0.196
78 0.307 0.299 0.303 0.138
39 0.236 0.229 0.233 0.068
0 0.167 0.162 0.165  
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Function Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
Subcellular Location Secreted
Protein Families Protease inhibitor I35 (TIMP) family
Tissue Specificity Ubiquitously expressed. Highest expression in kidney and ovary.
Database Links

KEGG: cfa:403816

STRING: 9615.ENSCAFP00000022333

UniGene: Cfa.3680

Pathway HIF-1 signaling pathway

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