Dog Transferrin receptor protein 1(TFRC) ELISA kit

Instructions
Code CSB-EL023441DO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name transferrin receptor (p90, CD71)
Alternative Names TFRC ELISA Kit; Transferrin receptor protein 1 ELISA Kit; TR ELISA Kit; TfR ELISA Kit; TfR1 ELISA Kit; Trfr ELISA Kit; CD antigen CD71 ELISA Kit
Abbreviation TFRC
Uniprot No. Q9GLD3
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog TFRC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 98
Range % 94-102
1:400 Average % 96
Range % 92-99
1:800 Average % 102
Range % 98-104
1:1600 Average % 87
Range % 82-93
Recovery
The recovery of dog TFRC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 86-97
EDTA plasma (n=4) 94 90-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
10 0.089 0.091 0.090
5 0.112 0.118 0.115
2.5 0.148 0.154 0.151
1.25 0.210 0.222 0.216
0.625 0.353 0.367 0.360
0.312 0.651 0.665 0.658
0.156 0.958 1.014 0.986
0 1.838 1.921 1.880
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Positively regulates T and B cell proliferation through iron uptake (By similarity).
Gene References into Functions
  1. sTfR significantly inhibited CPV infection of cultured feline F81 cells and decreased the mortality of CPV-infected dogs, which indicates that the sTfR has the anti-CPV activity both in vitro and in vivo PMID: 24089666
  2. The kinesin KIF16B mediates apical transcytosis of transferrin receptor in AP-1B-deficient epithelia. PMID: 23749212
  3. binds to CPV-2 or CPV-2b but only to very low levels, and does not bind FPV capsids PMID: 16912298
  4. comparison of the abilities of TfR1 orthologs from different species to support arenavirus entry found that the human and feline receptors were able to enhance entry of the pathogenic strains, but that neither the murine or canine forms were functional. PMID: 18003730
Subcellular Location Cell membrane, Single-pass type II membrane protein, Melanosome
Protein Families Peptidase M28 family, M28B subfamily
Database Links

KEGG: cfa:403703

STRING: 9615.ENSCAFP00000019067

UniGene: Cfa.33569

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