Dog complement fragment 3a(C3a) ELISA kit

Code CSB-EQ027542DO
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Product Type ELISA kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation C3a
Target Name complement fragment 3a(C3a)
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 ng/mL-1600 ng/mL
Sensitivity 6.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 81-89
1:2 Average % 95
Range % 92-99
1:4 Average % 86
Range % 83-89
1:8 Average % 94
Range % 91-99
Recovery
The recovery of dog C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 85-90
EDTA plasma (n=4) 104 101-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
1600 1.911 1.968 1.940 1.778
800 1.632 1.602 1.617 1.455
400 0.959 0.989 0.974 0.812
200 0.561 0.598 0.580 0.418
100 0.302 0.297 0.300 0.138
50 0.246 0.251 0.249 0.087
25 0.194 0.196 0.195 0.033
0 0.161 0.163 0.162  
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