Fish Interleukin 1β (IL- 1β) ELISA Kit

Instructions
Code CSB-E13259Fh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Interleukin 1β (IL- 1β)
Alternative Names il1bInterleukin-1 beta ELISA Kit
Abbreviation IL1B
Uniprot No. Q9YGD3
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish IL- 1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 82-89
1:2 Average % 94
Range % 90-99
1:4 Average % 104
Range % 97-108
1:8 Average % 99
Range % 94-106
Recovery
The recovery of fish IL- 1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-102
EDTA plasma (n=4) 89 82-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.989 2.778 2.884 2.776
100 2.467 2.432 2.450 2.342
50 1.774 1.866 1.820 1.712
25 0.956 0.932 0.944 0.836
12.5 0.436 0.456 0.446 0.338
6.25 0.258 0.270 0.264 0.156
3.12 0.156 0.155 0.156 0.048
0 0.105 0.111 0.108  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Gene References into Functions
  1. Major changes due to graded levels of olive leaf extract in the diets were observed in blood biochemical parameters. TNFalpha, IL1-beta and IL-8 gene expressions were significanlty up-regulated in OLE 0.1% group compared with others. PMID: 29733961
  2. The predicted 3D structures of IL-1beta, TNF-alpha, and IL-8 from Oncorhynchus mykiss, Dicentrarchus labrax, and Chionodraco hamatus are reported and the differences between them and their mammalian counterparts investigated. PMID: 21184658
Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families IL-1 family
Tissue Specificity All three isoforms are expressed in gill, kidney, liver and spleen. Isoform 1 is also expressed in blood. None of the isoforms are expressed in brain.
Database Links

UniGene: Omy.11696

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