Goat Beta-Endorphin(β-EP) ELISA Kit

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Code CSB-E13306G
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation β-EP
Target Name Beta-Endorphin(β-EP)
Species Capra hircus (Goat)
Sample Types serum, plasma
Detection Range 4000 pg/mL-20 pg/mL
Sensitivity 10 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 94    
Range % 90-100    
1:2 Average % 93    
Range % 86-98    
1:4 Average % 89    
Range % 85-97    
1:8 Average % 93    
Range % 86-99    
Recovery
The recovery of goat β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 94 90-99    
EDTA plasma (n=4) 96 90-102    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average      
0 2.568 2.541 2.555      
20 1.413 1.376 1.395      
80 0.958 0.986 0.972      
320 0.472 0.502 0.487      
1000 0.367 0.355 0.361      
4000 0.153 0.157 0.155      
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