Goat prostaglandin E2 (PGE2) ELISA kit

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Code CSB-EQ027602GO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation PGE2
Target Name prostaglandin E2 (PGE2)
Species Capra hircus (Goat)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.4 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat PGE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 82-93
1:2 Average % 106
Range % 103-110
1:4 Average % 98
Range % 94-102
1:8 Average % 93
Range % 90-96
Recovery
The recovery of goat PGE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 85-90
EDTA plasma (n=4) 94 89-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
6000 2.522 2.598 2.560 2.455
3000 2.049 2.068 2.059 1.954
1500 1.621 1.523 1.572 1.467
750 1.088 1.098 1.093 0.988
375 0.710 0.765 0.738 0.633
187.5 0.422 0.402 0.412 0.307
93.75 0.280 0.264 0.272 0.167
0 0.106 0.104 0.105  
References
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