Guinea Pig Interleukin 1(IL-1) ELISA Kit

Instructions
Code CSB-E15959Gp
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Pig Interleukin 1(IL-1)
Alternative Names IL1AInterleukin-1 alpha ELISA kit; IL-1 alpha ELISA kit; Fragment ELISA kit
Abbreviation IL-1
Uniprot No. Q60480
Species Cavia porcellus (Guinea pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 89-99
1:2 Average % 102
Range % 96-107
1:4 Average % 98
Range % 92-104
1:8 Average % 86
Range % 81-92
Recovery
The recovery of guinea pig IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 97-106
EDTA plasma (n=4) 86 81-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.734 2.875 2.805 2.716
100 2.112 2.246 2.179 2.090
50 1.434 1.412 1.423 1.334
25 0.828 0.808 0.818 0.729
12.5 0.446 0.455 0.451 0.362
6.25 0.237 0.246 0.242 0.153
3.12 0.168 0.173 0.171 0.082
0 0.088 0.089 0.089  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells (By similarity).
Subcellular Location Secreted
Protein Families IL-1 family
Database Links

STRING: 10141.ENSCPOP00000016482

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