Guinea pig Growth hormone,GH ELISA Kit

Code CSB-E06770Gu
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9JKM4
Lead Time 3-5 working days
Abbreviation GH1
Target Name growth hormone 1
Alias GH, GH-N, GHN, IGHD1B, hGH-N, pituitary growth hormone
Species Guinea Sus scrofa (Pig)
Sample Types serum, plasma
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.31 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 88-96    
1:2 Average % 84    
Range % 80-88    
1:4 Average % 97    
Range % 92-101    
1:8 Average % 90    
Range % 85-94    
Recovery
The recovery of guinea pig GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 100 96-107    
EDTA plasma (n=4) 88 84-92    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
80 2.374 2.428 2.401 2.251    
40 2.019 2.162 2.091 1.941    
20 1.680 1.593 1.637 1.487    
10 1.046 0.975 1.011 0.861    
5 0.658 0.681 0.670 0.520    
2.5 0.427 0.439 0.433 0.283    
1.25 0.265 0.254 0.260 0.110    
0 0.153 0.147 0.150      
References
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Function Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues (By similarity).
Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

STRING: 10141.ENSCPOP00000016097

Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway

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