Horse Annexin A1(ANXA1) ELISA kit

Instructions
Code CSB-EL001836HO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name annexin A1
Alternative Names ANXA1 ELISA Kit; ANX1Annexin A1 ELISA Kit; Annexin I ELISA Kit; Annexin-1 ELISA Kit; Calpactin II ELISA Kit; Calpactin-2 ELISA Kit; Lipocortin I ELISA Kit
Abbreviation ANXA1
Uniprot No. Q8HZM6
Species Equus caballus (Horse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of horse ANXA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 85-100
1:2 Average % 95
Range % 85-102
1:4 Average % 93
Range % 85-99
1:8 Average % 90
Range % 86-98
Recovery
The recovery of horse ANXA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 86-99
EDTA plasma (n=4) 94 87-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
800 2.502 2.535 2.519 2.382
400 1.926 1.965 1.946 1.809
200 1.253 1.285 1.269 1.132
100 0.785 0.793 0.789 0.652
50 0.471 0.486 0.479 0.342
25 0.325 0.332 0.329 0.192
12.5 0.226 0.230 0.228 0.091
0 0.135 0.138 0.137  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Promotes resolution of inflammation and wound healing (By similarity). Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (By similarity). Has no effect on unstimulated T-cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (By similarity). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (By similarity). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).
Subcellular Location Nucleus, Cytoplasm, Cell projection, cilium, Basolateral cell membrane, Lateral cell membrane, Cell membrane, Peripheral membrane protein, Apical cell membrane, Membrane, Peripheral membrane protein, Early endosome, Cytoplasmic vesicle membrane, Peripheral membrane protein, Endosome membrane, Peripheral membrane protein, Secreted, Secreted, extracellular space, Cell membrane, Peripheral membrane protein, Extracellular side, Secreted, exosome, Cytoplasmic vesicle, secretory vesicle lumen, Cell projection, phagocytic cup
Protein Families Annexin family
Database Links

KEGG: ecb:100033940

STRING: 9796.ENSECAP00000013992

UniGene: Eca.12920

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