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Horse Interleukin-12 subunit beta(IL12B) ELISA kit

Horse Interleukin-12 subunit beta(IL12B) ELISA kit

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Code	CSB-EL011587HO
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Product Details

Target Name

interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

Alternative Names

IL12B ELISA Kit; Interleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit

Abbreviation

IL12B

Uniprot No.

Q9XSQ5

Species

Equus caballus (Horse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

39 pg/mL-2500 pg/mL

Sensitivity

9.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse IL12B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	97
	Range %	94-103
1:8	Average %	101
	Range %	96-105

Recovery

The recovery of horse IL12B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	94-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.862	2.988	2.925	2.824
1250	2.234	2.355	2.295	2.194
625	1.483	1.512	1.498	1.397
312.5	0.830	0.854	0.842	0.741
156.25	0.447	0.457	0.452	0.351
78.12	0.275	0.280	0.278	0.177
39	0.199	0.201	0.200	0.099
0	0.097	0.105	0.101

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

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Target Data

Function	Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Subcellular Location	Secreted
Protein Families	Type I cytokine receptor family, Type 3 subfamily
Database Links	KEGG: ecb:100034222 STRING: 9796.ENSECAP00000010803 UniGene: Eca.12460

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Horse Interleukin-12 subunit beta(IL12B) ELISA kit

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