Horse Interleukin-12 subunit beta(IL12B) ELISA kit

Instructions
Code CSB-EL011587HO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Alternative Names IL12B ELISA Kit; Interleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
Abbreviation IL12B
Uniprot No. Q9XSQ5
Species Equus caballus (Horse)
Sample Types serum, plasma, tissue homogenates
Detection Range 39 pg/mL-2500 pg/mL
Sensitivity 9.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of horse IL12B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 88-95
1:2 Average % 95
Range % 90-100
1:4 Average % 97
Range % 94-103
1:8 Average % 101
Range % 96-105
Recovery
The recovery of horse IL12B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-94
EDTA plasma (n=4) 96 94-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.862 2.988 2.925 2.824
1250 2.234 2.355 2.295 2.194
625 1.483 1.512 1.498 1.397
312.5 0.830 0.854 0.842 0.741
156.25 0.447 0.457 0.452 0.351
78.12 0.275 0.280 0.278 0.177
39 0.199 0.201 0.200 0.099
0 0.097 0.105 0.101  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Subcellular Location Secreted
Protein Families Type I cytokine receptor family, Type 3 subfamily
Database Links

KEGG: ecb:100034222

STRING: 9796.ENSECAP00000010803

UniGene: Eca.12460

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