Horse Tumor necrosis factor α,TNF-α ELISA Kit

Code CSB-E14031Hs
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name tumor necrosis factor (TNF superfamily, member 2)
Alternative Names TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Abbreviation TNF
Uniprot No. P29553
Species Equus caballus (Horse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of horse TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %86
Range %80-97
1:2Average %92
Range %84-99
1:4Average %95
Range %92-103
1:8Average %92
Range %86-98
Recovery
The recovery of horse TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9283-98
EDTA plasma (n=4)9491-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.601 2.548 2.575 2.409
5002.248 2.211 2.230 2.064
2501.631 1.616 1.624 1.458
1250.905 0.925 0.915 0.749
62.50.475 0.483 0.479 0.313
31.20.312 0.321 0.317 0.151
15.60.255 0.268 0.262 0.096
00.164 0.167 0.166
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Gene References into Functions
  1. The results obtained showed that IL-6 expression in adipose tissue biopsies derived from animals with equine metabolic syndrome was enhanced while TNF-alpha levels of both groups were comparable. PMID: 25269712
  2. Data indicate that tumor necrosis factor-alpha production is closely related to ovarian steroid actions. PMID: 24642167
  3. These results indicate that in equine corpus luteum, cytokines TNF, IFNG and FASL regulate nitric oxide activity, via eNOS expression modulation. PMID: 23941776
  4. These data show the presence of cytokines TNF and IFNG, and their receptors, in the equine corpus luteum and indicate their potential involvement in regulation of luteal function. PMID: 22186103
  5. These results suggest that basal oxidative stress markers, circulating cytokines and anti-inflammatory neuroendocrine hormones appear to correlate with endurance performance in horses. PMID: 21058993
  6. The contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of IL-6, IL-10 and TNF-alpha, in horses with recurrent airway obstruction during exacerbation and in remission is reported. PMID: 18294392
  7. The effects of semen extender and seminal plasma on the expression of inflammatory modulators in the endometrium of mares are reported. PMID: 18584861

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Subcellular Location Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Protein Families Tumor necrosis factor family
Database Links

KEGG: ecb:100033834

STRING: 9796.ENSECAP00000001460

UniGene: Eca.13019

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