Human 1,25-dihydroxyvitamin D3 (DVD/DHVD3) ELISA kit

Code CSB-E05120h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation DVD/DHVD3
Target Name 1,25-dihydroxyvitamin D3
Species Homo sapiens (Human)
Sample Types serum
Detection Range 1000 fmol/L-5000 fmol/L
Sensitivity 250 fmol/L
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision

 

Intra-assay Precision (Precision within an assay): CV%<10%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of Human1,25-dihydroxyvitamin D3 (1,25 DVD/DHVD3)in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

References
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