Human 25-hydroxy vitamin D3,25HVD3 ELISA Kit

Code CSB-E08097h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation 25 HVD3
Protein Biological Process 1 Metabolism
Target Name 25-hydroxy vitamin D3,25HVD3
Species Homo sapiens (Human)
Sample Types serum
Detection Range 20 μg/L-100 μg/L
Sensitivity 5 μg/L
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<10%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human 25HVD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 92  
Range % 85-99  
1:10 Average % 91  
Range % 89-100  
1:20 Average % 95  
Range % 88-103  
1:40 Average % 92  
Range % 83-98  
Recovery
The recovery of human 25HVD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/L OD1 OD2 Average    
100 0.306 0.287 0.297    
80 0.453 0.475 0.464    
40 0.933 0.934 0.934    
20 1.345 1.381 1.363    
0 2.160 2.272 2.216    
References
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