Human 4F2 cell-surface antigen heavy chain(SLC3A2) ELISA kit

Unavailable
Code CSB-EL021640HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P08195
Lead Time 5-7 working days
Abbreviation SLC3A2
Protein Biological Process 1 Transport
Target Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Alias 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, 4F2 cell-surface antigen heavy chain|4F2 heavy chain|CD98 heavy chain|antigen defined by monoclonal antibody 4F2, heavy chain|antigen identified by mono
Species Homo sapiens (Human)
Protein Biological Process 3 Amino-acid transport
Sample Types serum, plasma, tissue homogenates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.72 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein with an alpha amylase domain. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. It associates with integrins and mediates integrin-dependent signaling related to normal cell growth and tumorigenesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
HGNC 11026
RGD 3073
MGI 96955
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SLC3A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 85-95
1:2 Average % 102
Range % 94-107
1:4 Average % 90
Range % 83-95
1:8 Average % 98
Range % 90-102
Recovery
The recovery of human SLC3A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 81-94
EDTA plasma (n=4) 92 85-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 1.998 1.926 1.962 1.802
1500 1.426 1.495 1.461 1.301
750 1.098 1.134 1.116 0.956
375 0.788 0.741 0.765 0.605
187.5 0.547 0.532 0.540 0.380
93.75 0.374 0.359 0.367 0.207
46.88 0.297 0.285 0.291 0.131
0 0.162 0.158 0.160  
References
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Function Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. When associated with LAPTM4B, recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation
Subcellular Location Apical cell membrane, Single-pass type II membrane protein, Melanosome
Protein Families SLC3A transporter family
Tissue Specificity Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Exp
Database Links

HGNC: 11026

OMIM: 158070

KEGG: hsa:6520

STRING: 9606.ENSP00000367123

UniGene: Hs.502769

Pathway mTOR signaling pathway

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