Human A disintegrin and metalloproteinase with thrombospondin motifs 12(ADAMTS12) ELISA kit

Instructions
Code CSB-EL001300HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ADAM metallopeptidase with thrombospondin type 1 motif, 12
Alternative Names ADAMTS12 ELISA Kit; UNQ1918/PRO4389A disintegrin and metalloproteinase with thrombospondin motifs 12 ELISA Kit; ADAM-TS 12 ELISA Kit; ADAM-TS12 ELISA Kit; ADAMTS-12 ELISA Kit; EC 3.4.24.- ELISA Kit
Abbreviation ADAMTS12
Uniprot No. P58397
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:20 Average % 93
Range % 86-97
1:40 Average % 102
Range % 95-106
1:80 Average % 89
Range % 84-92
1:160 Average % 100
Range % 91-104
Recovery
The recovery of human ADAMTS12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 95-107
EDTA plasma (n=4) 92 85-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.889 2.789 2.839 2.742
1500 2.476 2.348 2.412 2.315
750 1.768 1.724 1.746 1.649
375 1.011 1.045 1.028 0.931
187.5 0.485 0.477 0.481 0.384
94 0.302 0.321 0.312 0.215
47 0.184 0.176 0.180 0.083
0 0.093 0.101 0.097  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties.
Gene References into Functions
  1. ADAMTS 1, 4, 12, and 13 levels in the maternal and cord blood were lower in the preeclampsia group than in the control group. ADAMTS 1, 4, and 12 levels in placental tissues were higher in the preeclampsia group. PMID: 29135310
  2. ADAMTS12 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  3. Decreased levels of ADAMTS-12 were found to be associated with intrahepatic cholestasis of pregnancy. PMID: 26363034
  4. ADAMTS-12 is a multifaced metalloproteinase in arthritis and inflammation [review] PMID: 24876675
  5. ADAMTS-12 is implicated in the inflammatory response by modulating normal neutrophil apoptosis. PMID: 23019333
  6. Our results suggest that ADAMTS12 may be a susceptibility gene for RA development. PMID: 22505177
  7. statistically significant increase in mRNA expression of ADAMTS-7 and ADAMTS-12 was observed in the endplate cells in degenerative discs compared with nondegenerative discs PMID: 22247065
  8. The data supported the hypothesis that genetic variations in ADAMTS12 influence the risk of schizophrenia. PMID: 22322903
  9. expression of ADAMTS12 in colorectal cancer stroma plays an important role in inhibiting tumor development PMID: 21559743
  10. This study identifies a novel biological role for ADAMTS-12, and highlights the importance and complexity of its non-proteolytic domain(s) pertaining to its function. PMID: 21494557
  11. ADAMTS-12 is a new COMP-interacting and -degrading enzyme and thus may play an important role in the COMP degradation in the initiation and progression of arthritis PMID: 16611630
  12. data suggest that ADAMTS12 confers tumour-protective functions upon cells that produce this proteolytic enzyme PMID: 17895370
  13. ADAMTS-7 and ADAMTS-12 are newly identified enzymes responsible for cartilage oligomeric matrix protein degradation in arthritis. PMID: 19098927
  14. Results demonstrate that ADAMTS-12, a downstream molecule of PTHrP signaling, is a novel regulator of chondrogenesis. PMID: 19151918
  15. ADAMTS-12 is a novel anti-tumor protease that can reduce the proliferative properties of tumor cells. PMID: 19638407

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Subcellular Location Secreted, extracellular space, extracellular matrix
Tissue Specificity Expressed in skeletal muscle and fat. Detected at significant levels in fetal lung. Widely expressed in gastric carcinomas and in cancer cells of diverse origin.
Database Links

HGNC: 14605

OMIM: 606184

KEGG: hsa:81792

STRING: 9606.ENSP00000422554

UniGene: Hs.12680

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