Human Alpha-1B-glycoprotein(A1BG) ELISA kit

Instructions
Code CSB-EL001001HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name alpha-1-B glycoprotein
Alternative Names A1B ELISA Kit; A1BG ELISA Kit; A1BG_HUMAN ELISA Kit; ABG ELISA Kit; Alpha 1 B glycoprotein ELISA Kit; Alpha-1-B glycoprotein ELISA Kit; Alpha-1B-glycoprotein ELISA Kit; DKFZp686F0970 ELISA Kit; Epididymis secretory sperm binding protein Li 163pA ELISA Kit; GAB ELISA Kit; HEL S 163pA ELISA Kit; HYST2477 ELISA Kit
Abbreviation A1BG
Uniprot No. P04217
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 ng/mL-4000 ng/mL
Sensitivity 15.6 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human A1BG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 89
Range % 82-95
1:200 Average % 95
Range % 90-99
1:400 Average % 97
Range % 91-103
1:800 Average % 93
Range % 87-98
Recovery
The recovery of human A1BG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 98-107
EDTA plasma (n=4) 93 86-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
4000 2.637 2.584 2.611 2.528
2000 2.126 2.195 2.161 2.078
1000 1.443 1.494 1.469 1.386
500 0.872 0.889 0.881 0.798
250 0.433 0.441 0.437 0.354
125 0.251 0.261 0.256 0.173
62.5 0.137 0.144 0.141 0.058
0 0.082 0.083 0.083  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Gene References into Functions
  1. cardiovascular outcomes may differ based on A1BG genotype, and antihypertensive treatment strategy. PMID: 23690342
  2. Data indicate that five important proteins, vimentin, gelsolin, alpha 2 HS glycoprotein (AHSG), glial fibrillary acidic protein (GFAP), and alpha1B-glycoprotein (A1BG) were expressed higher in Rheumatoid arthritis (RA) synovial fluid than non-RA samples. PMID: 23418544
  3. Overexpression of serum A1BG is associated with non-small cell lung cancer. PMID: 23284758
  4. an 11-fold upregulated 13.8 kDa fragment of alpha 1-B glycoprotein (A1BG) as a biomarker for steroid-resistant nephrotic syndrome PMID: 21591266
  5. Complex binding of CRISP proteins by A1BG and other proteins may interfere with the detection and function of these proteins. PMID: 20116414
  6. A1BG-cysteine-rich secretory protein 3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP-3 PMID: 15461460
  7. MMP-9, DJ-1 and A1BG proteins are overexpressed in pancreatic juice from pancreatic ductal adenocarcinoma PMID: 18706098

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Subcellular Location Secreted
Tissue Specificity Plasma.
Database Links

HGNC: 5

OMIM: 138670

KEGG: hsa:1

STRING: 9606.ENSP00000263100

UniGene: Hs.529161

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