Human Annexin Ⅲ(ANX-Ⅲ) ELISA Kit

Code CSB-E12157h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P12429
Lead Time 3-5 working days
Abbreviation ANXA3
Target Name annexin A3
Alias ANX3, Annexin III (lipocortin III)|annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha)|calcimedin 35-alpha
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.
HGNC 541
RGD 2119
MGI 1201378
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅲin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %92-101
1:2Average %90
Range %82-96
1:4Average %101
Range %99-104
1:8Average %87
Range %82-99
Recovery
The recovery of human ANX-Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9082-96
EDTA plasma (n=4)9589-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.425 2.550 2.488 2.370
252.010 2.174 2.092 1.974
12.51.266 1.363 1.315 1.197
6.250.559 0.597 0.578 0.460
3.120.275 0.261 0.268 0.150
1.560.192 0.186 0.189 0.071
0.780.131 0.140 0.136 0.018
00.122 0.113 0.118  
References
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Function Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
Protein Families Annexin family
Database Links

HGNC: 541

OMIM: 106490

KEGG: hsa:306

STRING: 9606.ENSP00000264908

UniGene: Hs.480042

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