Human Annexin Ⅲ(ANX-Ⅲ) ELISA Kit

Instructions
Code CSB-E12157h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name annexin A3
Alternative Names ANX3, Annexin III (lipocortin III)|annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha)|calcimedin 35-alpha
Abbreviation ANXA3
Uniprot No. P12429
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅲin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %92-101
1:2Average %90
Range %82-96
1:4Average %101
Range %99-104
1:8Average %87
Range %82-99
Recovery
The recovery of human ANX-Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9082-96
EDTA plasma (n=4)9589-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.425 2.550 2.488 2.370
252.010 2.174 2.092 1.974
12.51.266 1.363 1.315 1.197
6.250.559 0.597 0.578 0.460
3.120.275 0.261 0.268 0.150
1.560.192 0.186 0.189 0.071
0.780.131 0.140 0.136 0.018
00.122 0.113 0.118  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
Protein Families Annexin family
Database Links

HGNC: 541

OMIM: 106490

KEGG: hsa:306

STRING: 9606.ENSP00000264908

UniGene: Hs.480042

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