Human Annexin Ⅲ(ANX-Ⅲ) ELISA Kit

Instructions
Code CSB-E12157h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name annexin A3
Alternative Names 2-cyclic phosphate 2-phosphohydrolase ELISA Kit; 35-alpha calcimedin ELISA Kit; Annexin A3 ELISA Kit; Annexin III ELISA Kit; Annexin-3 ELISA Kit; ANX3 ELISA Kit; ANXA 3 ELISA Kit; Anxa3 ELISA Kit; ANXA3_HUMAN ELISA Kit; Calcimedin 35 alpha ELISA Kit; Inositol 1 2 cyclic phosphate 2 phosphohydrolase ELISA Kit; Inositol 1 ELISA Kit; Lipocortin III ELISA Kit; OTTHUMP00000160701 ELISA Kit; OTTHUMP00000219237 ELISA Kit; OTTHUMP00000219238 ELISA Kit; PAP III ELISA Kit; PAP-III ELISA Kit; Placental anticoagulant protein III ELISA Kit
Abbreviation ANXA3
Uniprot No. P12429
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅲin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %92-101
1:2Average %90
Range %82-96
1:4Average %101
Range %99-104
1:8Average %87
Range %82-99
Recovery
The recovery of human ANX-Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9082-96
EDTA plasma (n=4)9589-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.425 2.550 2.488 2.370
252.010 2.174 2.092 1.974
12.51.266 1.363 1.315 1.197
6.250.559 0.597 0.578 0.460
3.120.275 0.261 0.268 0.150
1.560.192 0.186 0.189 0.071
0.780.131 0.140 0.136 0.018
00.122 0.113 0.118  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
Gene References into Functions
  1. ANXA3 levels in urine show clinically significant correlation with real tumor volumes. PMID: 28703915
  2. The down-regulation of Annexin A3 by siRNA inhibited the invasion and epithelial-mesenchymal transition of colorectal cancer cells through the up-regulation of p53. PMID: 29224019
  3. ANXA3 plays important roles in gastric cancer carcinogenesis and metastasis. PMID: 27894078
  4. The findings implicate the expression of ANXA3 with the natural progression of breast cancer and associate it with increased lymphatic metastasis. The study validates the use of ANXA3 as a potential prognosis biomarker for breast cancer. PMID: 28497041
  5. we identified ANXA3 as a regulator of hepatitis C virus maturation and egress PMID: 27653686
  6. ANXA3 role in the proliferation and invasion of breast cancer cells. PMID: 27878264
  7. Results identified potential variant in ANXA3 gene [chr4, c.C820T(p.R274*)] in a large family with an unknown equinus deformity, which could lead to a three-dimensional conformational change. PMID: 27475959
  8. Anxa3 knockdown inhibited the growth, migration, invasion, and metastasis of lung adenocarcinoma. PMID: 27995049
  9. These results suggest that the iEA index or a combination of polymorphisms in EGFR and ANXA3 may serve as predictive factors of drug response, and therefore could be useful for optimal selection of chemotherapy regimens. PMID: 26475168
  10. ANX A3 has roles as a mammary biomarker, regulator and therapeutic target in breast cancer PMID: 26093083
  11. Our results suggest that ANXA3 can serve as a novel diagnostic biomarker and that the inhibition of ANXA3 may be a viable therapeutic option for the treatment of CD133+ liver-CSC-driven HCC. PMID: 26095609
  12. Findings reveal that ANXA3 might play an important role in hepatocellular carcinoma progression and chemoresistance. PMID: 24375474
  13. findings suggest that ANXA3 plays a role in HCC CSC/CIC maintenance, and that ANXA3 may represent a potential CSC/CIC-specific therapeutic target for improving the treatment of HCC. PMID: 25267273
  14. Two different antigenic variants of ANXA3 are present in post-DRE urines and their clinical significance for diagnosis of prostate cancer should be further investigated. PMID: 24954692
  15. Expression of annexin A3 was increased in gastric cancer compared with that in normal gastric tissues. Annexin A3 expression was significantly associated with tumor volume and TNM stage. PMID: 24815437
  16. Annexin A3 was upregulated in gastric cancer cells. Deletion of endogenous Annexin A3 significantly inhibited gastric cancer cell proliferation, migration, and invasion. PMID: 24824926
  17. Low ANXA3 expression is associated with radioresistance in nasopharyngeal carcinoma. PMID: 23464856
  18. Annexin A3 expression correlates with tumor size and lymph node metastasis.Annexin A3 might be regulated apoptosis by affecting Bcl-2/Bax balance.Annexin A3 was an independent prognostic factor in breast cancer. PMID: 23631820
  19. The expression change of Anxa3 can be utilized as a potential indicator for the development, invasion, metastasis and drug resistance of tumors. PMID: 23011854
  20. the association of multi-drug resistance with ANXA3, one of the highly expressed proteins in BEL7402/5-FU-resistant hepatoma cell line, was verified PMID: 22189913
  21. annexin A3 secretion may be associated with exocytosis and the release of exosomes PMID: 21435174
  22. Decreased expression of ANXA3 in papillary thyroid cancer supports the idea that ANXA3 may be an effective marker of microcarcinoma, and a negative predictor of papillary thyroid cancer progression. PMID: 21137070
  23. AnnexinA3 plays an important role in the initiation and progression of human gallbladder cancer. PMID: 21055154
  24. two spliced isoforms of Annexin A3 are expressed differently in human renal cortex and renal-cell carcinoma PMID: 20167856
  25. Annexin A3 could be a target for therapeutic intervention and may also serve as a biomarker for drug resistance in ovarian cancer patients. PMID: 20103635
  26. ANXA3 is a novel angiogenic factor that induces vascular endothelial growth factor production through the hypoxia-inducible factor-1 pathway PMID: 16236264

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Protein Families Annexin family
Database Links

HGNC: 541

OMIM: 106490

KEGG: hsa:306

STRING: 9606.ENSP00000264908

UniGene: Hs.480042

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