Human Annexin Ⅱ(ANX-Ⅱ) ELISA Kit

Instructions
Code CSB-E12156h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name annexin A2
Alternative Names Annexin A2 ELISA Kit; Annexin II ELISA Kit; Annexin II; heavy chain ELISA Kit; Annexin-2 ELISA Kit; ANX 2 ELISA Kit; ANX2 ELISA Kit; ANX2L4 ELISA Kit; ANXA2 ELISA Kit; ANXA2_HUMAN ELISA Kit; arylsulfatase B ELISA Kit; CAL1H ELISA Kit; Calpactin I heavy chain ELISA Kit; calpactin I heavy polypeptide (p36) ELISA Kit; Calpactin I heavy polypeptide ELISA Kit; Calpactin-1 heavy chain ELISA Kit; chromobindin 8 ELISA Kit; Chromobindin-8 ELISA Kit; Epididymis secretory protein Li 270 ELISA Kit; HEL S 270 ELISA Kit; LIP2 ELISA Kit; Lipocortin II ELISA Kit; LPC2 ELISA Kit; LPC2D ELISA Kit; p36 ELISA Kit; P36 protein ELISA Kit; PAP-IV ELISA Kit; Placental anticoagulant protein IV ELISA Kit; Protein I ELISA Kit
Abbreviation ANXA2
Uniprot No. P07355
Species Homo sapiens (Human)
Sample Types serum, urine, saliva, cell lysates
Detection Range 0.39 ng/mL-25 ng/mL
Sensitivity 0.098 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-94
1:2Average %84
Range %80-88
1:4Average %97
Range %92-101
1:8Average %103
Range %96-110
Recovery
The recovery of human ANX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
252.748 2.812 2.780 2.659
12.52.131 2.176 2.154 2.033
6.251.491 1.404 1.448 1.327
3.120.866 0.839 0.853 0.732
1.560.546 0.524 0.535 0.414
0.780.363 0.391 0.377 0.256
0.390.254 0.269 0.262 0.141
00.125 0.117 0.121
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9
Gene References into Functions
  1. Mechanistic studies revealed that FOXD2AS1 upregulated the expression of the miR206 target gene ANXA2 in hepatocellular carcinoma by acting as a miR206 sponge. PMID: 30272362
  2. study has localised AnxA2/S100A10 complexes to key anatomical locations in the placenta and suggests a role for this complex in amniotic epithelium, trophoblasts and syncytium, in addition to its well-known roles in endothelial cells PMID: 30143909
  3. Inhibition of ANXA2 will affect the expression of downstream targets and downstream signaling pathways, leading to the suppression of the malignant progression of tumors. PMID: 30355917
  4. our data suggest that overexpression of peroxiredoxin-2, annexin A2, and heat shock protein beta-1 was correlated with tumor invasion, metastasis, and poor prognosis, and therefore, these proteins may serve as potential diagnostic and therapeutic biomarkers. PMID: 29332450
  5. aptamer wh6 can block MM cell adhesion to ANXA2 and block the proliferation of MM cells induced by ANXA2 PMID: 29906496
  6. The results demonstrated that ANX2 and stromal tenascin C regulated invasion in addition to stemness and anoikis resistance, which are crucial for metastasis in the progression of pancreatic ductal adenocarcinoma. PMID: 29749431
  7. highly expressed in gastric cancer, related to tumor size, histological differentiation, lymph node metastasis PMID: 29097873
  8. models for annexin A2-mediated vesicle aggregation mechanisms. PMID: 29567212
  9. Our profiling data provided a rich source for further study of the molecular mechanisms of acquired drug resistance inneuroblastoma ( NB). Further study may determine the role of ANXA2 as a prognostic biomarker and a potential therapeutic target for patients with multidrug-resistant NB. PMID: 28814318
  10. These results suggested that increased ANXA2 and less expressed beta-catenin were correlated to adenomyosis-associated dysmenorrhea PMID: 28547742
  11. These data suggested that Annexin A2 induces cisplatin resistance of non-small cell lung cancer (NSCLC)via regulation of JNK/c-Jun/p53 signaling, and provided an evidence that blockade of Annexin A2 could serve as a novel therapeutic approach for overcoming drug resistance in NSCLCs PMID: 28886730
  12. Results show that Anxa2-Tyr23 phosphorylation is necessary for proliferation, invasion, and metastasis of breast cancer cells in vitro and in vivo. Tyr23-phosphorylated Anxa2 binds to STAT3 and serves as a key regulator of STAT3 signaling. PMID: 28470457
  13. annexin A2 can potentially predict the development of pre-eclampsia. The low annexin A2 levels (<0.89 ng/ml) together with higher blood pressure and proteinuria constitute a higher risk to develop preeclampsia. PMID: 28501283
  14. Variants in the Annexin A2 (ANXA2) gene directly influence plasma LDL-C levels. PMID: 28456096
  15. this study showed that Annexin A2 inhibition suppresses proliferation and invasion in ovarian cancer via beta-catenin/EMT, proposing the potential role of Annexin A2 in the prevention and treatment of ovarian cancer PMID: 28440436
  16. FNDC3B and ANXA2 expression correlate negatively with patient survival in hepatocellular carcinoma PMID: 27385217
  17. UBAP2 formed a complex with Annexin A2 and promoted the degradation of Annexin A2 protein by ubiquitination, and then inhibited HCC progression. PMID: 27121050
  18. results indicate that ANXA2 plays an important role to enhance the malignant behaviors of Hepatocellular carcinoma cells, and the enhancement is closely based on its remodeling to cell structures PMID: 27060670
  19. Thus, tight regulation of AnxA2 function is an integral aspect of cellular homeostasis. The presence of AnxA2 in cancer cell-derived exosomes, as well as the potential regulation of exosomal AnxA2 by phosphorylation or other PTMs, are topics of great interes PMID: 28867585
  20. Restoration of miR-101 expression reversed chemoresistance of drug-resistant gastric cancer cells by inhibiting viability and enhancing apoptosis via targeting ANXA2. PMID: 28609840
  21. Annexin 2A (ANXA2) is over-expressed in Glioblastoma (GBM) and positively correlates with patient outcome. PMID: 27429043
  22. Results confirm that both circRNA expression and even ANXA2 expression could be used as biomarkers of RR-MS with good values of specificity and sensitivity. PMID: 28651352
  23. This study demonstrated that the expression level of AnxA2 was significantly higher in recurrent adamantinomatous craniopharyngioma compared with primary ones. AND AnxA2(+) adamantinomatous craniopharyngioma cells exhibited enhanced proliferation and migration ability compared with AnxA2(-) adamantinomatous craniopharyngioma cells. PMID: 27640198
  24. Cercvical cancer stem cells express annexin II, an HPV co-receptor. PMID: 27008711
  25. Annexin A2 (ANXA2) and prostate-specific antigen (PSA) proteins measured from biopsy tumour regions are unlikely to be good biomarkers for prediction of the clinical outcome of prostate cancer presenting with apparently localized disease. PMID: 29187477
  26. anti-ANXA2 antibodies play a role in thrombotic mechanisms leading to recurrent pregnancy loss and placental vascular disease PMID: 27631133
  27. results suggest that mycoplasma induces a resistance to multiple drugs in hepatocarcinoma cells which required the interaction of P37 and Annexin A2. The pathway downstream this interaction needs to be explored PMID: 28976984
  28. Findings indicate that Munc13-4 supports acute WPB exocytosis by tethering WPBs to the plasma membrane via AnxA2-S100A10. PMID: 28450451
  29. Detecting ANXA2 and ANXA4 expression may aid the evaluation of cervical carcinoma prognosis. PMID: 27402115
  30. our studies show that this G-motif represents a novel and essential determinant for axonal localization of the Anxa2 mRNA mediated by the SMN complex. PMID: 28258160
  31. The data data suggest that interactions between HIV-1 gp120 and A2 exist, though this interaction may be indirect. PMID: 27863502
  32. Exosome-associated Annexin II plays an important role in angiogenesis and breast cancer metastasis. PMID: 27760843
  33. ANXA2 stromal expression might play a key role in aggressive tumor phenotype associated with increased EMT CTCs release, however, other factors beyond ANXA2 are responsible for coagulation activation mediated by CTCs in breast cancer patients. PMID: 28476852
  34. annexin A2 contributes to lung injury and fibrotic disease by mediating the fibrogenic actions of FXa. PMID: 28283478
  35. Annexin A2 and HB-EGF are overexpressed and are being secreted into serum in Her-2 negative breast cancer patients. PMID: 27496793
  36. results indicate that ANXA2 facilitates PS-ASO trafficking from early to late endosomes where it may also contribute to PS-ASO release PMID: 27378781
  37. Annexins A2 and A8 in endothelial cell exocytosis and the control of vascular homeostasis PMID: 27451994
  38. Rack1 regulated P-Glycoprotein activity, which was necessary for adriamycin-induced P-Glycoprotein - mediated phosphorylation of Anxa2 and Erk1/2. PMID: 27754360
  39. The presence of vascular lesions in lupus nephritis (LN) appears to be associated with significant differences in the vascular expression of ANXA2. PMID: 26511441
  40. expression elevated in lung squamous cell carcinoma tissue PMID: 28178129
  41. annexin A2 but not follistatin is expressed in hepatocellular carcinoma PMID: 26189841
  42. Our results place ANXA2 at the apex of a regulatory cascade that determines glioblastoma mesenchymal transformation and validate aSICS as a general methodology to uncover regulators of cancer subtypes. PMID: 27667176
  43. Overexpression of ANXA2 is associated with colorectal cancer. PMID: 27468721
  44. Annexin A2, up-regulated by IL-6, promotes ligament ossification in ankylosing spondylitis patients. PMID: 27697640
  45. highly specific 30 kDa plant lectin could dissociate AnxA2 from endogenous lectin galectin-3 interaction at the cell surface. This dissociation could down-regulate Bcl-2 family proteins, cell proliferation, and migration simultaneously triggering cell apoptosis PMID: 26438086
  46. Data show that ONECUT2, IGF2BP1, and ANXA2 proteins were confirmed to be microRNA-9 (miR-9) targets and aberrantly upregulated in hepatocellular carcinoma (HCC). PMID: 26547929
  47. annexin A2 and S100A10 expressions are powerful predictors of serous ovarian cancer outcome. PMID: 26925708
  48. expression of ANXA2 is associated with tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma PMID: 26196246
  49. Anxa2 binds to STAT3 and promotes epithelial to mesenchymal transition in breast cancer. PMID: 26307676
  50. the role of Axna2 is instructive for understanding the development of RA, suppress the effect of Axna2 might provide a new potential measure for treatment of RA. PMID: 26963384

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Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane, Melanosome
Protein Families Annexin family
Database Links

HGNC: 537

OMIM: 151740

KEGG: hsa:302

STRING: 9606.ENSP00000346032

UniGene: Hs.511605

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