Human Apolipoprotein A-I-binding protein(APOA1BP) ELISA kit

Instructions
Code CSB-EL001914HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein A-I binding protein
Alternative Names NAXE ELISA Kit; AIBP ELISA Kit; APOA1BP ELISA Kit; YJEFN1 ELISA Kit; NAD(P)H-hydrate epimerase ELISA Kit; EC 5.1.99.6 ELISA Kit; Apolipoprotein A-I-binding protein ELISA Kit; AI-BP ELISA Kit; NAD(P)HX epimerase ELISA Kit; YjeF N-terminal domain-containing protein 1 ELISA Kit; YjeF_N1 ELISA Kit
Abbreviation APOA1BP
Uniprot No. Q8NCW5
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, urine, saliva
Detection Range 0.7 pg/mL-500 pg/mL
Sensitivity 0.7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human APOA1BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 96
Range % 90-101
1:2000 Average % 95
Range % 89-105
1:4000 Average % 90
Range % 82-100
1:8000 Average % 90
Range % 86-100
Recovery
The recovery of human APOA1BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-100
EDTA plasma (n=4) 97 91-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 1.865 1.849 1.857 1.725
167 1.675 1.662 1.669 1.537
56 1.259 1.273 1.266 1.134
18 0.964 0.949 0.957 0.825
6 0.613 0.586 0.600 0.468
2 0.376 0.365 0.371 0.239
0.7 0.232 0.230 0.231 0.099
0 0.135 0.129 0.132  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.
Gene References into Functions
  1. NAXE gene mutations are associated with early-onset progressive encephalopathy. PMID: 30022751
  2. Study shows a homozygous mutation in the APOA1BP is associated with a lethal infantile leukoencephalopathy.This is the first report of a defect in the nicotinamide nucleotide repair system in humans. PMID: 27122014
  3. NAXE Mutations Disrupt the Cellular NAD(P)HX Repair System and Cause a Lethal Neurometabolic Disorder of Early Childhood PMID: 27616477
  4. AIBp contributes to mitotic entry and bipolar spindle assembly. PMID: 26114227
  5. AIBP accelerates cholesterol efflux from endothelial cells to HDL and thereby regulates angiogenesis PMID: 23719382
  6. Taken together, the current study demonstrates that APN might protect against atherosclerosis by increasing HDL assembly through enhancing ABCA1 pathway and apoA-1 synthesis in the liver. PMID: 17521614

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Involvement in disease Encephalopathy, progressive, early-onset, with brain edema and/or leukoencephalopathy (PEBEL)
Subcellular Location Mitochondrion, Secreted
Protein Families NnrE/AIBP family
Tissue Specificity Ubiquitously expressed, with highest levels in kidney, heart and liver. Present in cerebrospinal fluid and urine but not in serum from healthy patients. Present in serum of sepsis patients (at protein level).
Database Links

HGNC: 18453

OMIM: 608862

KEGG: hsa:128240

STRING: 9606.ENSP00000357218

UniGene: Hs.528320

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