Human Aquaporin 5,AQP-5 ELISA Kit

Instructions
Code CSB-E08257h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name aquaporin 5
Alternative Names AQP 5 ELISA Kit; AQP-5 ELISA Kit; AQP5 ELISA Kit; AQP5_HUMAN ELISA Kit; Aquaporin 5 ELISA Kit; Aquaporin-5 ELISA Kit
Abbreviation AQP5
Uniprot No. P55064
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %87-96
1:2Average %98
Range %95-103
1:4Average %98
Range %94-105
1:8Average %94
Range %90-95
Recovery
The recovery of human AQP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-99
EDTA plasma (n=4)9993-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
103.014 2.951 2.983 2.847
52.465 2.545 2.505 2.369
2.51.802 1.905 1.854 1.718
1.251.132 1.060 1.096 0.960
0.6250.625 0.659 0.642 0.506
0.3120.406 0.428 0.417 0.281
0.1560.237 0.255 0.246 0.110
00.133 0.139 0.136
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity
Gene References into Functions
  1. Results indicate that autophagy plays a crucial role in aquaporin 5 (AQP5) degradation in diabetic submandibular gland (SMG). PMID: 29951954
  2. these results suggest that AQP5 silencing enhances the sensitivity of colorectal cancer (CRC) cells to 5-FU, and the underlying mechanism is related to inhibition of the Wnt-beta-catenin pathway. AQP5 could be a useful therapeutic target for CRC treatment. PMID: 29390193
  3. Structural basis for mutations in human AQP5 associated with keratoderma has been described. PMID: 29799470
  4. The urea transporter subtypes, UT-A1 and UT-B1, were expressed in the skin basal cell layer and exocrine sweat glands. The abundance of UT-A1 and UT-B1 in uremic sweat glands was significantly increased in UP, while the expression of AQP5 was decreased. PMID: 29279852
  5. we demonstrated that AQP5 gene silencing could inhibit the proliferation, reduce the migration and promote the apoptosis of human glioma cells by suppressing EGFR/ERK/p38 MAPK signaling pathway PMID: 28404978
  6. Data suggest that urine AQP5/creatinine ratio is significantly higher in patients with diabetic nephropathy than in control subjects, subjects diabetes, or subjects with nephropathy of unknown etiology; urine AQP5/creatinine ratio increases with stage of diabetic nephropathy; this biomarker may improve clinical models in distinguishing diabetic nephropathy from normal controls and subjects with type 2 diabetic alone. PMID: 27103565
  7. We present the first Danish family diagnosed with autosomal dominant palmplantar ketratoderma of Bothnian type resulting from c.562C>T, p.Arg188Cys in the AQP5 gene. PMID: 27255181
  8. Findings show that overexpression of aquaporin 5 (AQP5) activated epithelial-mesenchymal transition (EMT) in colorectal cancer (CRC) cells. PMID: 28833571
  9. RAQP5 protein expression in breast cancer.Rac1 is a potential downstream signaling partner of AQP5 in breast cancer. PMID: 28958942
  10. The AQP5 genotype may influence survival following lipopolysaccharides by altering neutrophil cell migration. PMID: 27871297
  11. Mucins and AQP5 gene expression were significantly higher in patients with OME relative to controls. A 2-fold increase in AQP5 correlated with increased effusion viscosity (1.94 mL/mg; 95% CI, 0.08-3.80 mL/mg). PMID: 28594978
  12. The severity of preterm infants with acute respiratory distress syndrome was associated with the plasma level of AQP5; the more severe of the disease, the higher levels of plasma AQP5. PMID: 25877715
  13. AQP5 is up-regulated in hepatocellular carcinoma (HCC) cell line. AQP5 promotes hepatocellular carcinoma metastasis via NF-kappa B-regulated epithelial-mesenchymal transition. PMID: 28619511
  14. Double IF showed the co-localization of AQP5 and LC3B on BafA1-treated heated cells. In conclusion, we demonstrated that heat shock decreased AQP5 on cellular membranes and in the cytoplasm by activating autophagic degradation, and heat shock and AQP5 knockdown exerted similar anticancer effects, suggesting that heat shock exerts anticancer effects via the autophagic degradation of AQP5. PMID: 28358429
  15. we analyzed the urine excretion of AQP5 and AQP2 via exosomes, in 35 diabetic patients: 12 normoalbuminuric with normal renal function (DM), 11 with proteinuric nondiabetic nephropathy (NDN), and 12 with histological diagnosis and classification of DN. urinary AQP5 and AQP2 were significantly increased in DN patients. PMID: 28246612
  16. this study shows that S-allylmercapto-l-cysteine can suppress AQP5 secretion using the cell model of chronic obstructive pulmonary disease PMID: 27517516
  17. The current study suggests AQP5 rs1964676 as a new potential prognostic marker in patients with EBC involved in AQP5 expression PMID: 27978515
  18. Adjusting AQP5 protein levels could be considered a therapeutic strategy for the treatment of acute pulmonary edema induced by H2S and other hazardous gases PMID: 28088675
  19. nuclear matrix protein 4 overexpression increased Aquaporin 5 mRNA expression by 2.5-fold in HEK293 cells PMID: 27058007
  20. The findings presented here add further support to mutations in AQP5 being responsible for this particular subtype of NEPPK and also have implications for the optimal initial genetic screening of other British individuals with this clinical diagnosis. PMID: 26032342
  21. AQP5 plasma membrane abundance in transfected HEK293 cells is rapidly and reversibly regulated by at least three independent mechanisms involving phosphorylation at Ser156, protein kinase A activity and extracellular tonicity. PMID: 26569106
  22. AQP5 can be both overexpressed and lost in subgroups of prostate cancers PMID: 26614400
  23. AQP5 defined a subset of patients with Bcl-2-negative and p16-negative tumours with a poor clinical outcome. PMID: 26074259
  24. Histamine downregulates AQP5 production in human nasal epithelial cells by inhibiting cyclic adenosine monophosphate-responsive element binding protein (CREB) phosphorylation at serine 133. PMID: 25781725
  25. Aquaporin-5 is expressed in adipocytes with implications in adipose differentiation. PMID: 25631586
  26. These results indicate that NFAT5 plays important roles in proliferation and migration of human lung adenocarcinoma cells through regulating AQP5 expression, providing a new therapeutic option for lung adenocarcinoma therapy. PMID: 26299924
  27. The hyperosmotic induction of AQP5 and VEGF in retinal pigment epithelial cells was in part dependent on activation of NFAT5. PMID: 25878490
  28. AQP5 was strongly localized in the apical membrane and weakly localized in the cytoplasm of secretory epithelial cells. PMID: 25218052
  29. The AQP5 protein is up-regulated in prostate cancer and is closely related to advanced stage, lymph node metastasis, and poor prognosis. AQP5 expression was associated with cell proliferation and migration. PMID: 25217331
  30. In colorectal adenocarcinoma, 31.1% had high levels of expression of AQP5, 64.4% exhibited a moderate level of staining, and 4.4% had an absence of AQP5 staining. AQP5 was only occasionally detected in para-neoplastic (6.67%) and normal tissues (6.67%). PMID: 25109507
  31. Down-regulated aquaporin 5 inhibits proliferation and migration of human epithelial ovarian cancer 3AO cells. PMID: 25298246
  32. These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function. PMID: 22715407
  33. Survival of patients with a high AQP-5/PTEN coexpression was longer than that of patients with a low coexpression (p = 0.003). PMID: 24217644
  34. High AQP5 expression is associated with breast cancer. PMID: 24114055
  35. So these results indicate that AQP5 is associated with drug resistance of colon cancer, and that the AQP5-P38 MAPK pathway may represent a potential drug target to improve drug resistance of colon cancer cells. PMID: 24752576
  36. We described a Chinese family with palmoplantar keratoderma Bothnia type in which we identified a gain-of-function mutation in AQP5. PMID: 23867895
  37. By directing cellular localization of TRPC1 and AQP5 channels and by selectively regulating the functional assembly TRPC1-STIM1 channels, Cav1 is a crucial determinant of SG fluid secretion. PMID: 23203809
  38. AQP5-L51R does not exhibit the H2O or CO2 permeability of the wild-type protein. PMID: 23842530
  39. Mutations in AQP5 cause autosomal-dominant diffuse nonepidermolytic palmoplantar keratoderma. PMID: 23830519
  40. The regulated AQP5 translocation may contribute to sweat secretion by increasing the water permeability of apical plasma membranes of sweat glands. PMID: 23473857
  41. AQP5 polymorphism A(-1364)C is associated with peritumoral brain edema in meningioma patients PMID: 23392848
  42. Our findings clearly indicate that binding of anti-M3R autoantibodies to the receptor, which was verified by immunoprecipitation, suppresses AQP5 trafficking to the membrane and contribute to impaired fluid secretion in SjS. PMID: 23382834
  43. Overexpression of AQP5 is associated with lymphatic Metastasis in gastric carcinoma. PMID: 23436048
  44. These observations suggest that AQP5 plays a key role in cervical cancer PMID: 22048942
  45. These observations suggest that sialadenosis is associated with a different AQP5 expression and distribution pattern in salivary acinar cells. PMID: 23160677
  46. A lipid, phosphatidylserine, is bound to AQP5 in the central pore, totally occluding it and inhibits the function of the central pore with an IC(50) in the micromolar range. PMID: 23176748
  47. The variant G allele of AQP5 polymorphism rs3736309 reduces the risk of Meniere's disease. PMID: 23352976
  48. AQP5 is a significant component of lens fiber cell membranes, representing the second most abundant water channel in these cells. PMID: 23313152
  49. The potential functions of AQP2 and AQP5 are in the resorption and secretion of endolymph. (Review) PMID: 22732097
  50. TNF-alpha inhibition of AQP5 expression in human salivary gland acinar cells is due to the epigenetic mechanism by suppression of acetylation of histone H4. PMID: 21973049
  51. the results of this study suggest that AQP-5 expression may be associated with prognosis and drug sensitivity in biliary tract carcinoma PMID: 22470085
  52. Numbers of AQP-5-positive coils in the secretory part of eccrine sweat glands were decreased in Sjogren's syndrome, but not in skin affected by idiopathic segmental anhidrosis or idiopathic pure sudomotor failure. PMID: 21950762
  53. AQP5 overexpression is associated with cell growth and metastasis of human breast cancer. PMID: 22145049
  54. Aquaporin-5, which mediates water permeability and thus regulates cell shape and volume, is a potentially important determinant in cell migration. PMID: 21315850
  55. Treatment of A549 cells with tanshinone IIA can decrease the overexpression of AQP5 induced by seawater. PMID: 21251364
  56. AQP5-IR was found on apical and basolateral membranes of acinar cells, and showed a "mosaic" pattern, i.e., with some acini and/or acinar cells showing stronger AQP5-IR than others. PMID: 21524183
  57. AQP5 expression increases MUC5AC and MUC5B mucin production PMID: 21455588
  58. When inhibiting the proliferation of ovarian cancer cells, topotecan may down-regulate the expression of AQP5 and NF-kappaB. PMID: 19664333
  59. AQP5, HIF-1alpha, VEGF are all involved in edema formation in nasal polyps. PMID: 20669661
  60. Aquaporin 5 polymorphisms have a role in rate of lung function decline in chronic obstructive pulmonary disease PMID: 21151978
  61. AQP5 was expressed more highly in patients with nasal polyps and allergic rhinitis than in patients with nasal polyps alone. PMID: 19166033
  62. Aquaporin 5 gene promoter--1364A/C polymorphism associated with 30-day survival in severe sepsis. PMID: 21427539
  63. AQP5 transcription and protein expression were down-regulated after exposure to lipopolysaccharide via the p38/JNK signaling pathway. PMID: 20362698
  64. Up-regulation of AQP5 may be involved in differentiation of human gastric cancer cells. PMID: 19340551
  65. This is the first reliable investigation of the regulation of MUC5AC mucin secretion by silencing AQP5. PMID: 16500622
  66. TRPV4 participates in hypotonic reduction of AQP5. PMID: 16537379
  67. hyperosmotic stress is an important activator of AQP-5 in human airway epithelium, leading to significantly increased transepithelial water permeability PMID: 17043812
  68. These results show that IL-13 interferes with ion and water channel expression and localization during epithelial regeneration and may thereby influence mucus composition and hydration. PMID: 17553491
  69. The decreased expression of AQP5 mRNA may be related to the severity of airflow obstruction. PMID: 17640479
  70. We conclude that AQP5 membrane targeting can include more than one mechanism besides cAMP dependent phosphorylation. PMID: 18042467
  71. elements found in the aquaporin-5 and the Rad9 (radiation-sensitive 9) genes showed selective AR versus GR binding in band-shift assays and a strong activity and selectivity in functional assays, both as isolated elements and in their original contexts PMID: 18215141
  72. AQP5 expression was needed for shear-induced barrier enhancement PMID: 18305162
  73. Genetic background differences or a distinct pathogenetic Keratoconus (KC) cascade specific to the analyzed population could account for the dissimilarities observed in KC-related AQP5 expression. PMID: 18449356
  74. AQP5, through its phosphorylation on Ser156 and subsequent interaction with c-Src, plays an important role in NSCLC invasion and, therefore, may provide a unique opportunity for developing a novel therapeutic target as well as a prognostic marker in NSCLC PMID: 18478076
  75. There is an induction of hAQP5 expression during colorectal carcinogenesis. hAQP5 ingteracts with the Ras/extracellular signal-regulated kinase/retinoblastoma protein signaling pathway, identifying hAQP5 as a novel therapeutic target. PMID: 18583321
  76. AQP5 is overexpressed in chronic myelogenous leukemia cells and plays a role in promoting cell proliferation and inhibiting apoptosis. PMID: 18612408
  77. C terminus may arise from the phosphorylation of AQP5 and thereby signal trafficking PMID: 18768791
  78. A single nucleotide polymorphism in the AQP5 gene promoter alters AQP5 expression in different in vitro systems and cells, and is associated with alterations of variables of the RAAS both in young healthy males and patients with coronary artery disease. PMID: 18846354
  79. The +2254A to G variant in intron 3 of AQP5 was associated with a decreased risk of COPD in a Chinese population. PMID: 18853286
  80. expressed in primary human lung parenchyma mesenchymal stromal cells PMID: 19652646

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Involvement in disease Keratoderma, palmoplantar, Bothnian type (PPKB)
Subcellular Location Apical cell membrane, Multi-pass membrane protein
Protein Families MIP/aquaporin (TC 1.A.8) family
Database Links

HGNC: 638

OMIM: 600231

KEGG: hsa:362

STRING: 9606.ENSP00000293599

UniGene: Hs.298023

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