Human Beta-1 adrenergic receptor(ADRB1) ELISA kit

Instructions
Code CSB-EL001391HU
Size 96T,5×96T,10×96T
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Product Details

Target Name adrenergic, beta-1-, receptor
Alternative Names ADR B1 ELISA Kit; ADRB 1 ELISA Kit; ADRB1 ELISA Kit; ADRB1_HUMAN ELISA Kit; ADRB1R ELISA Kit; Adrenergic beta 1 receptor ELISA Kit; Adrenoceptor beta 1 ELISA Kit; B1AR ELISA Kit; Beta 1 adrenoceptor ELISA Kit; Beta 1 adrenoreceptor ELISA Kit; Beta-1 adrenergic receptor ELISA Kit; Beta-1 adrenoceptor ELISA Kit; Beta-1 adrenoreceptor ELISA Kit; BETA1AR ELISA Kit; RHR ELISA Kit
Abbreviation ADRB1
Uniprot No. P08588
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ADRB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 87-98
1:2 Average % 97
Range % 91-103
1:4 Average % 92
Range % 87-98
1:8 Average % 87
Range % 81-92
Recovery
The recovery of human ADRB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 98-109
EDTA plasma (n=4) 92 86-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 1.765 1.875 1.820 1.642
800 1.412 1.522 1.467 1.289
400 1.013 1.045 1.029 0.851
200 0.708 0.717 0.713 0.535
100 0.443 0.459 0.451 0.273
50 0.315 0.333 0.324 0.146
25 0.241 0.247 0.244 0.066
0 0.177 0.179 0.178  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling.
Gene References into Functions
  1. There was no association of SNPs in ADRB1, GRK5 and BAG3 genes with Takotsubo cardiomyopathy. PMID: 29514624
  2. These results suggested that adrenergic stimulation via beta1- and beta2-adrenergic receptors in the human endolymphatic sac was involved in regulation of inner ear fluid ion homeostasis PMID: 28165045
  3. Arg389Gly polymorphism in ADRB1 is not associated with overweight, obesity or dyslipidaemias in Saudis PMID: 29587766
  4. This study provided proof that SNPs within ADRB1 is significantly associated with the susceptibility to and the prognosis of cervical vertigo, so that NPY and NA might serve as the precise diagnostic marker and treatment target of cervical vertigo. PMID: 29197114
  5. Report associations between ADRB1 SNPs and acute coronary syndrome/cardiovascular risk factors in Arab population. PMID: 26602751
  6. The downregulation of beta-ARs after ureter dilation, particularly for beta1-AR and beta3-AR in the muscular layer, suggests a potential compensatory mechanism involving increased contraction of the ureter to push urine through the obstruction. PMID: 28756611
  7. The results demonstrate that Gly/Gly polymorphism in Arg389Gly ADRB1 was an independent risk factor for hypertension; moreover, the patients who carried the Gly389Gly genotype had a significantly improved metoprolol antihypertensive effect than those with ADRB1. PMID: 29549925
  8. All patients carrying the Val158-COMT allele exhibited higher preoperative norepinephrine concentrations. Moreover, we found that both beta1-adrenoceptor polymorphisms were associated with a longer stay in hospital. We conclude that patients with the Val158-COMT genotype exhibit higher endogenous resting plasma norepinephrine levels. PMID: 28522796
  9. two barcodes are involved in sorting Wild Type ss1-AR out of early endosomes. PMID: 27816670
  10. For adrenergic receptors, a potential dimer interface involving transmembrane domain 1 (TMD1) and helix 8 (H8) was identified in crystal structures of the beta1-adrenergic (beta1-AR) and beta 2-AR PMID: 27993566
  11. Data suggest that the partial agonist STD-101-D1 of beta-1 adrenergic receptor (ADRB1) is an research tool to study mechanisms of G protein-coupled receptor signal transduction. PMID: 28746336
  12. Analyzing the functional relevance of individual sites using phosphosite-deficient receptor mutants we found phosphorylation of the ADRB1 at Ser461/Ser462 in the distal part of the C-terminus to determine beta-arrestin2 recruitment and receptor internalization PMID: 28472170
  13. Studied frequency of ADRB1 Arginine389Glycine (Arg389Gly) and Cytochrome p450 2D6*10 (Cyp2D6*10) polymorphism in cases of heart failure-reduced ejection fraction (HFREF), and to evaluate the influence of the polymorphisms in response to beta-blocker (BB) therapy; found a statistically significant association was observed with CC genotype and Glisin-Glisin (GG) genotype. PMID: 27665326
  14. Selective alpha1-adrenergic receptor blockade provoked disturbances in the distribution of regional cerebral blood flow that resulted in significant changes in ipsilateral hemispheric blood flow and conductance during static handgrip exercise. PMID: 27016578
  15. Among individuals with previous small artery ischemic stroke, the ADRB1 Gly49 polymorphism was associated with major adverse cardiovascular events, particularly small artery ischemic stroke, a risk that may be increased among beta-blocker-treated individuals. PMID: 28351962
  16. ADRB1 SNPs were associated with myocardial infarction susceptibility, blood pressure and plasma lipids. PMID: 28456594
  17. the beta adrenoreceptor Gly 49 allele of the beta1 -adrenergic receptor Ser(49) Gly polymorphisms may increase the risk of implantable cardioverter-defibrillators shock in patients with heart failure, independent of beta-blocker dosage PMID: 27027728
  18. This study demonstrates that the polypeptide GalNAc-transferase 2 (GalNAc-T2) specifically O-glycosylates beta 1-adrenergic receptor at five residues in the extracellular N terminus, including the Ser-49 residue at the location of the common S49G single-nucleotide polymorphism. PMID: 28167537
  19. The minor alleles of ADRB1 and ADRB3 were significantly underrepresented in kinesiology students compared with nonmajors. PMID: 28177749
  20. The ADRB1 Gly389X genotype showed greater response to bisoprolol than the Arg389Arg genotype, suggesting the potential of individually tailoring beta-blocker therapy according to genotype in Korean heart failure patients. PMID: 26879662
  21. The multiplex SNaPshot method allows for specific and accurate detection of CYP2D6 genotypes and ADRB1 genotypes and haplotypes. This platform is simple and efficient and suited for high throughput. PMID: 27108086
  22. Suggest a common regulatory pattern for the beta(1)-AR/GRK5 which is independent of cellular type or pathology. PMID: 26248277
  23. In healthy individuals, heart rate and renin responses to dobutamine were more than three-fold greater among ADRB1 Arg389 compared with Gly389 homozygotes. PMID: 26313487
  24. ADRB1 is associated with heart rate recovery after exercise. PMID: 26058836
  25. Ser49Gly polymorphism of the ADRB1 gene confer higher risk of ischemic stroke in a North Indian population. PMID: 25510377
  26. the b1 adrenergic receptor gene Arg389Gly polymorphism might not be associated with heart failure risk PMID: 26125791
  27. There was no significant interaction between the effects of ADRB1 and CYP2D6 gene polymorphisms on treatment outcome. Patients homozygous for the mutant ADRB1 genotype (CC) had better treatment outcomes than those heterozygous for the mutation (GC). PMID: 25823457
  28. In women with mitral valve prolapse syndrome, symptoms at least partially may be related to beta1-adrenergic receptor polymorphism, which has been shown previously to be associated with a hyperresponse to adrenergic stimulation. PMID: 25172623
  29. This study sought to identify the distributed characteristics of these variant genotypes in Chinese population, and influence of GRK5 and ADRB1 polymorphisms on systolic heart failure morbidity. PMID: 25638254
  30. This evidence, in combination with clinical studies, may contribute to a detailed analysis of the pattern of susceptibility to CVD involved in b-ADR1 receptor mechanism failure. PMID: 25815601
  31. Higher serum anti-M2-R and anti-beta1-R levels are associated with lone paroxysmal atrial fibrillation. PMID: 25351416
  32. The variant Arg389Gly of ADRB1 was statistically associated with an increase of LDL PMID: 24972470
  33. negative cooperativity across a beta1-adrenoceptor homodimer may be responsible for generating the low-affinity pharmacology of the secondary beta1-adrenoceptor conformation PMID: 25837585
  34. beta2-Stimulation is, therefore, more arrhythmogenic than beta1-stimulation in heart failure. PMID: 25673629
  35. Our data suggest that PIST contributes to the fine-tuning of b1AR sorting both during biosynthetic and postendocytic trafficking PMID: 25614626
  36. Arg389 polymorphism of the ADRB1 gene and Gln27 polymorphism of the ADRB2 gene were associated with the hypotensive response to topic betaxolol in healthy Mexican volunteers. PMID: 24749907
  37. differences in interhelical interaction regulate the different activation speed and efficacy of ADRB1 variants PMID: 25451930
  38. The possible link between beta 1-AR Gly389Arg and Ser49Gly polymorphisms and echocardiography parameters in 165 normotensive patients with a thyrotoxicosis without any cardiovascular disorders, was investigated. PMID: 24982877
  39. There is a direct correlation between Heart Rate and Systolic blood pressure in hypertensive patients with overweight and obesity from polymorphism Arg389Gly of ADRbeta1 gene. PMID: 25558679
  40. Bucindolol prevented new-onset AF; beta and alpha(2c) polymorphisms predicted therapeutic response; and the 47% of patients who were beta389 Arg homozygotes had an enhanced effect size of 74%. PMID: 24159564
  41. The beta1 -AR Arg389/Gly polymorphism may be associated with increased risk of orthostatic hypotension in female hypertensive patients. PMID: 24552127
  42. Data indicate mutation of the beta1 adrenergic receptor (beta1AR) promotes sustained agonist-induced cAMP activity, protein kinase A (PKA) phosphorylation, and cardiac myocyte contraction response. PMID: 24713698
  43. Co-expression of COX2 and beta1AR lowers the expression of COX2 in a dose-dependent manner. Stimulation of the receptor does not reverse the beta1AR-induced decrease in COX2, suggesting that this effect does not occur via classical beta1-signaling pathways. PMID: 25091480
  44. High Beta1 adrenergic receptor expression is associated with peripartum cardiomyopathy. PMID: 24466231
  45. beta1 and beta3-adrenergic receptors were expressed in retinal endothelial cells while beta2-adrenergic receptor was not detectable both at protein and mRNA levels. PMID: 24885710
  46. The Arg389Gly polymorphism of the ADRB1 gene may be a worthy biological marker to predict the risk of developing cardiovascular diseases given a high-risk atherogenic index PMID: 24371822
  47. S49G and R389G polymorphisms of the beta-adrenergic receptor influence signaling via the cAMP-PKA and ERK pathways. PMID: 24151242
  48. Data did not support a relationship between the three polymorphisms of ADRB1 (rs1801253) and ADRB2 (rs1042713 and rs1042714) genes and risk of subsequent cardiovascular events after PCI in Han Chinese patients with CAD. PMID: 24342311
  49. ADRB1 genetic polymorphisms did not influence left ventricular reverse remodeling after cardiac resynchronization therapy. PMID: 23729404
  50. Our findings indicate a novel function for beta1AR-mediated beta-arrestin1 signaling activated by carvedilol in miR biogenesis. PMID: 24334028
  51. the nature of the residue at position -3 in the PDZ regulated whether the beta1-AR was internalized alone or in complex with SAP97. PMID: 24324269
  52. AJ45G polymorphism (Ser49Gly) at DNA sites encoding for the amino acid sequence of beta1- adrenoreceptors can influence the development of sex-specific cardiac rhythm disorders. PMID: 24437165
  53. The Gly389 variant in the beta1-adrenoreceptor is associated with postoperative atrial fibrillation. PMID: 24332148
  54. a significant role for the AKAP5 scaffold in signaling and trafficking of the beta1-AR in cardiac myocytes and mammalian cells. PMID: 24121510
  55. The Gly allele of ADRB1 Arg389Gly polymorphism might confer lower risk for essential hypertension, especially in East Asians. PMID: 23588958
  56. These results provide clarification to the logical candidacy of beta1-adrenoreceptor gene in the development of hypertension. PMID: 23271137
  57. We determined that in addition to uncoupling of the b1-AR from Gs, the A159E/I160R b1-AR did not phosphorylate ERK1/2. PMID: 23351074
  58. Report distinctions between infantile hemangioma and vascular malformations with regard to ADRB1 mRNA levels. PMID: 23370410
  59. beta1AR regulates the cAMP-binding protein Epac1 and consequently the activation of its downstream effectors Rap1 and Rap2B/Ras. PMID: 23266473
  60. Data suggest that ADRB1 Arg389Gly polymorphism may influence exercise tolerance in heart failure patients. PMID: 23065660
  61. There was a gene-dose interaction with the ADRbeta1-389 Arg/Arg vs. Gly carrier genotype and beta-blocker dose in heart failure patients. Arg/Arg genotype patients may need a higher dose to achieve a treatment response similar to that of Gly carriers. PMID: 23115322
  62. 389-Arg/Arg patients with heart failure and atrial fibrillation had nominally significant reductions in all-cause mortality/HF hospitalization and cardiovascular mortality/hospitalization with bucindolol, whereas Gly carriers did not. PMID: 23223178
  63. PCR revealed that chronic atrial fibrillation up-regulated beta1-Adrenoceptor expression PMID: 23060133
  64. results suggest that SNPs in the ADRB1 gene are associated with individual differences in pain and analgesic sensitivity PMID: 23257656
  65. Up-regulation of beta1AR action was eliminated with 17beta-estradiol addition to the media, directly implicating this hormone as a regulator of beta1AR expression. PMID: 23533220
  66. The early effect of esmolol on exercise-induced tachycardia was significantly greater among Arg389 than in Gly389 homozygote healthy individuals. PMID: 23114278
  67. Beta1-adrenergic receptor polymorphisms are associated with cardiac dysfunction after brain death. PMID: 22994654
  68. the selected SNPs of beta(1)-AR and beta(2)-AR genes influence HR response to metoprolol in patients who undergo coronary computed tomographic angiography. PMID: 23261005
  69. beta-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines. PMID: 23318885
  70. Bucindolol prevents ventricular arrhythmias in subjects with heart failure and reduced left ventricular ejection fractions, and this effect is modulated by adrenergic beta(1) receptor polymorphisms. PMID: 23275278
  71. impact of beta1-AR polymorphisms on susceptibility to heart failure, the response to beta-blocker therapy and the prognosis PMID: 22815685
  72. genetic association studies in a population in Denmark: Data suggest that an SNP in ADRB1 (rs1801253) is associated with plasma renin activity (PRA) and PRA response to exercise or metoprolol (i.e., resistance to adrenergic beta-1 receptor blocker). PMID: 22703382
  73. Data indicate that disease-relevant beta1-adrenergic receptor (beta1-AR) autoantibodies in dilated cardiomyopathy (DCM) patient sera yielding a high reproducibility also in high throughput screening. PMID: 22811559
  74. beta-receptor gene variants significantly influence inotropic and chronotropic responses to beta-agonist exposure in patients on beta-blocker therapy. PMID: 22742717
  75. Through alpha(B)-D receptor heteromers dopamine inhibits adrenergic receptor signaling and blocks the synthesis of melatonin induced by adrenergic receptor ligands. PMID: 22723743
  76. Positivity for ADRB1 autoantibody predicts abnormal histological findings in 90% of idiopathic arrhythmia patients and suggests that autoimmunity might play an arrhythmogenic role. PMID: 22447021
  77. Prediction of radiographic severity in Ankolysing Spondylitis based on clinical variables can be significantly improved by including SNPs at ADRB1 (rs1801253), NELL1 (rs8176785) and MHC (rs1634747, rs9270986, rs7451962 and rs241453) genes. PMID: 22495925
  78. The heart rate-lowering effects of carvedilol in patients with heart failure plus atrial fibrillation are modulated by the Arg389Gly polymorphism of the beta1-adrenoceptor. PMID: 22617224
  79. beta-1 and beta-3 adrenergic receptors were expressed in an orthotopic mouse model of transduced Nalm-6 huma pre-B ALL cells PMID: 22306453
  80. Development of late hypertension after aortic coarctation repair could not be related to the investigated genomic polymorphism: the correlation of the ACE I/D and the ADRB1 c.1165C>G PMID: 20537417
  81. The Arg389Gly Arg/Arg genotype carriers are associated with an elevated risk of essential hypertension. PMID: 22340652
  82. the TGN acts as a checkpoint for both the recycling and down-regulation of beta1AR and arrestin-3 not only mediates beta1AR endocytosis but also its recycling through the TGN PMID: 22378779
  83. ADRB1 gene variation appears to impact stroke index and plasma norepinephrine levels but not heart rate. PMID: 21807569
  84. Nicotine-treated non-small cell lung cancer cells showed significant induction of the alpha7nAChR and alpha4nAChR, along with significant inductions of p-CREB and p-ERK1/2. PMID: 22253823
  85. Late positive potential amplitude depends on allelic variation in the beta1-receptor gene polymorphism. PMID: 21847570
  86. In this experimental model of Down syndrome, selective activation of beta1-ADR restores cerain behavioral deficits in a transgenic mouse model. PMID: 21527343
  87. Meta-analysis suggests no statistically significant association between beta1-adrenergic receptor gene polymorphisms and susceptibility to idiopathic dilated cardiomyopathy among Europeans. PMID: 21553224
  88. Report a significant interaction between ADRB1 Arg389Gly variant status and "beta-blocking" effect of selective serotonin reuptake inhibitors on heart rate and blood pressure. PMID: 20581797
  89. This study showed for the first time an association between takotsubo cardiomyopathy phenotype and beta1 adrenoreceptor gene polymorphisms. PMID: 22299391
  90. We have identified a common beta1-AR polymorphism, G389R, that is associated with adequate response to rate-control therapy in Atrial Fibrillation patients. PMID: 22192668
  91. In human multipotent adipose-derived stem cells differentiated into functional brown adipocytes, activation of either beta(1)-adrenergic receptors or beta(3)-adrenergic receptors was able to increase UCP1 mRNA and protein levels. PMID: 21878665
  92. The responsiveness to beta-blockers with respect to cardiac contractility is determined by the Arg389Gly beta(1)AR gene polymorphism. PMID: 21311897
  93. ADRB1 Gly49 was associated with higher baseline heart rates and with greater carvedilol effects on exercise heart rates. PMID: 21599570
  94. Results provide evidence that beta(2)-AR polymorphisms are significantly associated with the acceleration/deceleration pattern of heart rate oscillation, reflecting the underlying mode of autonomic nervous system control. PMID: 21573230
  95. Haplotype structures of common variants of ADRB1 genes in a South Indian population PMID: 21375401
  96. explores the relationship between LV remodeling after myocardial infarction and polymorphisms in the cardiac beta1-AR and beta2-AR genes PMID: 21626217
  97. The results of the present study do not support clinical use of genotyping for these polymorphisms to predict responses to beta-blockers. PMID: 21422729
  98. The ADRB1 1165C>G and 145A>G polymorphisms are not associated with the heart rate (HR), systolic (SBP) and diastolic (DBP) blood pressure responses in Polish Caucasian patients requiring diagnostic dobutamine stress echocardiography. PMID: 21305273
  99. Differential regulation of two palmitoylation sites in the cytoplasmic tail of the beta1-adrenergic receptor. PMID: 21464135
  100. There were associations of the polymorphism of ADRB1 gene (the Gly39Arg polymorphic locus) with the development and severity of CHF and with the efficacy of therapy with beta-adrenoblocker carvedilol. PMID: 21516734

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Subcellular Location Cell membrane, Multi-pass membrane protein, Early endosome
Protein Families G-protein coupled receptor 1 family, Adrenergic receptor subfamily, ADRB1 sub-subfamily
Database Links

HGNC: 285

OMIM: 109630

KEGG: hsa:153

STRING: 9606.ENSP00000358301

UniGene: Hs.99913

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