Human C-C Chemokine receptor 7,CCR-7 ELISA Kit

Instructions
Code CSB-E11293h
Size 96T,5×96T,10×96T
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Product Details

Target Name chemokine (C-C motif) receptor 7
Alternative Names BLR 2 ELISA Kit; BLR2 ELISA Kit; C C chemokine receptor type 7 ELISA Kit; C C CKR 7 ELISA Kit; C-C chemokine receptor type 7 ELISA Kit; C-C CKR-7 ELISA Kit; CC chemokine receptor 7 ELISA Kit; CC chemokine receptor type 7 ELISA Kit; CC CKR 7 ELISA Kit; CC-CKR-7 ELISA Kit; CCCKR7 ELISA Kit; CCR 7 ELISA Kit; CCR-7 ELISA Kit; Ccr7 ELISA Kit; CCR7_HUMAN ELISA Kit; CD 197 ELISA Kit; CD197 ELISA Kit; CD197 antigen ELISA Kit; CDw197 ELISA Kit; Chemokine (C C motif) receptor 7 ELISA Kit; Chemokine (C C) receptor 7 ELISA Kit; Chemokine C C motif receptor 7 ELISA Kit; Chemokine C C receptor 7 ELISA Kit; Chemokine receptor 7 ELISA Kit; Chemokine receptor 7-like protein ELISA Kit; CMKBR7 ELISA Kit; EBI 1 ELISA Kit; EBI1 ELISA Kit; Ebi1h ELISA Kit; EBV Induced G Protein Coupled Receptor 1 ELISA Kit; EBV-induced G-protein coupled receptor 1 ELISA Kit; Epstein Barr virus induced G protein coupled receptor ELISA Kit; Epstein Barr virus induced gene 1 ELISA Kit; Epstein-Barr virus-induced G-protein coupled receptor 1 ELISA Kit; EVI 1 ELISA Kit; EVI1 ELISA Kit; Lymphocyte Specific G Protein Coupled Peptide Receptor ELISA Kit; MGC108519 ELISA Kit; MIP 3 beta receptor ELISA Kit; MIP-3 beta receptor ELISA Kit; MIP3 Beta Receptor ELISA Kit
Abbreviation CCR7
Uniprot No. P32248
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCR-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 87-95  
1:2 Average % 98  
Range % 95-100  
1:4 Average % 97  
Range % 94-103  
1:8 Average % 94  
Range % 90-98  
Recovery
The recovery of human CCR-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-95  
EDTA plasma (n=4) 99 93-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1200 2.312 2.387 2.350 2.223  
600 1.468 1.535 1.502 1.375  
300 1.126 1.067 1.097 0.970  
150 0.670 0.710 0.690 0.563  
75 0.388 0.379 0.384 0.257  
37.5 0.246 0.255 0.251 0.124  
18.75 0.179 0.177 0.178 0.051  
0 0.126 0.128 0.127    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Gene References into Functions
  1. Results suggest that chemokine (C-C motif) receptor 7 (CCR7)promotes triple-negative breast cancer (TNBC) metastasis and may serve as a target for breast cancer diagnosis and treatment. PMID: 30032244
  2. High CCR7 expression is associated with urinary bladder cancer metastasis. PMID: 28534984
  3. these data indicate CCL19/CCR7 contributes to proliferation and invasion of ESCs, which are conducive to the pathogenesis of endometriosis through activating PI3K/Akt pathway PMID: 28856757
  4. The research findings demonstrate for the first time that the chemokines CCL19, CCL21 and CCR7 play important roles in bone destruction by increasing osteoclast migration and resorption activity, and that has been linked to rheumatoid arthritis pathogenesis. PMID: 28729639
  5. CXCR4, CCR7, VEGF-C and VEGF-D expression might have synergistic effects on the lymph node metastasis in patients with cervical cancer. PMID: 28535405
  6. the acute GvHD (aGvHD) patients received higher percentage of CD4+CCR7+ T-cells in donor T-cells, whereas chronic GvHD (cGvHD) patients were transplanted with higher percentages of CD8+CCR7+ T-cells. Functional experiments demonstrated that CCR7+ T-cells exhibited higher potential for activation than CCR7- T-cells did. PMID: 28112745
  7. these results demonstrated that CCL21/CCR7 may activate EMT in lung cancer cells via the ERK1/2 signaling pathway. PMID: 28487957
  8. CCL21/CCR7 interaction was shown to allow NK cell adhesion to endothelial cells (ECs) and its reduction by hypoxia. PMID: 28416768
  9. Study shows that miR-1275 can positively regulate CCR7 expression in squamous cell carcinoma of head and neck (SCCHN) with different mechanisms. PMID: 29278769
  10. Study provide evidence that CCR7 mediates EMT progress via AKT pathway, which indicates that CCR7 has a key role in breast cancer progression. PMID: 28378417
  11. Report an increased percentage of peripheral CCR7 T cells accompanied by endothelial dysfunction in patients with ankylosing spondylitis. PMID: 28421995
  12. The analysis of gene amplification and mRNA levels showed the expression of CCR7 in breast cancer correlated with better prognosis. PMID: 28930757
  13. that CCR7 mediates TGF-beta1-induced MMP2/9 expression through NF-kappaB signaling PMID: 28817313
  14. leukocyte subsets express distinct patterns of CCR7 sialylation that contribute to receptor signaling and fine-tuning chemotactic responses. PMID: 26819318
  15. tumor microenvironment stimulation down-regulated the migration of CCR7-expressing tumor cells toward CCL21 and inhibited the formation of directional protrusions toward CCL21 in a novel 3-dimensional hydrogel system. PMID: 26936935
  16. CCR7 expression levels in human tumors correlate with signatures of CD141(+) DC, intratumoral T cells, and better clinical outcomes. PMID: 27424807
  17. specific role for CCL21/CCR7 in promoting EMT and metastasis in CD133+ pancreatic cancer stem-like cells PMID: 27505247
  18. This study showed that CCR7 are overexpressed in CD4(-) CD8(-) thymocytes of myasthenia gravis patients. PMID: 26616645
  19. High tumoral CCR7 expression correlated with potential lymphatic involvement and poor prognosis of metastatic renal cell carcinoma patients treated with tyrosine kinase inhibitors. PMID: 28114889
  20. Results show that upregulation of CCR7 promotes cell proliferation and inflammation in A549 non-small cell lung cancer cells. However, silencing of CCR7 via siRNA treatment promotes cell apoptosis and suppresses the inflammatory response and TGF-beta1-induced EMT, which may be associated with NF-kappaB signaling. PMID: 28339080
  21. Down-regulating CCR7 expression in MG63 cells could apparently inhibit cell proliferation, migration and invasion abilities of MG63 cells, and also induce cell apoptosis. PMID: 27916085
  22. These results suggest that upregulation of rat CCR7 expression does not change the phenotype, differentiation, or proliferation capacity of human adipose-derived stem cells (hASCs), but does enable efficient migration of hASCs to rat secondary lymphoid organs. PMID: 28035134
  23. CCL21/CCR7 interaction contributes to the time-dependent proliferation of PTC cells by upregulating cyclin A, cyclin B1 and cyclin-dependent kinase 1 (CDK1) expression via the extracellular signal-regulated kinase (ERK) pathway associated with iodine. PMID: 27574129
  24. we conclude that CCR7 gene locus harbours a polymorphism that modifies risk of MI in patients with Coronary artery disease (CAD). Replication of this association could be sought in a prospective cohort of initially healthy individuals PMID: 27317472
  25. this study shows that plasmacytoid dendritic cells from rheumatoid arthritis patients have high expression levels of CCR7 PMID: 27421624
  26. This study suggests that NRP1 expression and LVD are independent factors that are likely to predict the risk of LN metastasis in squamous cell carcinoma (SCC)of the tongue, whereas the expression of VEGFC, VEGFR3, CCR7, and SEMA3E are nonindependent predictive factors PMID: 27666723
  27. High CCR7 expression is associated with gastric cancer. PMID: 26984468
  28. CCR7 pathway up-regulates Twist expression via ERK and PI3K/AKT signaling to manage the epithelial-mesenchymal transition of pancreatic ductal adenocarcinoma PMID: 26219899
  29. Constitutively expressed Siglec-9 inhibits LPS-induced CCR7, but enhances IL-4-induced CD200R expression in human macrophages. PMID: 26923638
  30. High CXCR4 expression in primary breast tumors (PTs) was found to be associated with luminal A type tumor, suggesting more favorable outcome. In contrast, CCR7 and FOXP3 expressions in PTs represented luminal B tumors, pointing to more aggressive tumor behavior. Maspin expression did not differ between luminal types. PMID: 28011488
  31. Results indicated that CCR7 is overexpressed in gallbladder cancer tissues and its expression correlates with staging and lymph node metastasis. PMID: 27009073
  32. CCL21/CCR7 induce VEGF-D up-regulation and promote lymphangiogenesis via ERK/Akt pathway in lung cancer. PMID: 26884842
  33. The present study demonstrated that down-regulation of CCR7 reduced proliferation, cell cycle, cell migration and invasion in prostate cancer cells PMID: 26722441
  34. Our findings suggested that MUC1 plays an important role in CCL21-CCR7-induced lymphatic metastasis and may serve as a therapeutic target in esophageal squamous cell carcinoma . PMID: 26667143
  35. Membrane associated PGES1dependent release of PGE2 is ccompanied by elevated CCR7 expression in colon cancer cells. PMID: 26352871
  36. Pyk2 is a key downstream signaling molecules of CCR7 in SCCHN, which promotes SCCHN tumorigenesis and progression. PMID: 26352169
  37. the CCR7 axis mediates TGF-beta1-induced EMT via crosstalk with NF-kappaB signaling, facilitating lymph node metastasis and poorer overall survival in patients with gastric cancer PMID: 26176983
  38. Blockade of CCR7, or treatment with a p38 MAP kinase inhibitor, reduced lymphatic dissemination of epithelial-mesenchymal transition cells. PMID: 25961925
  39. findings indicated that circulating memory Tfh cells, especially CCR7+ICOS+ memory Tfh cells, may be associated with the relapse of MS and may serve as a new therapeutic target PMID: 26231034
  40. The chemotactic interaction between CCR7 and its ligand, CCL21, may be a critical event during progression in pancreatic cancer PMID: 21594558
  41. CrkL regulates CCL19 and CCR7-induced epithelial-to-mesenchymal transition via ERK signaling pathway in epithelial ovarian carcinoma patients. PMID: 25636509
  42. Recycling accounts, to a major extent, for the high levels of surface CXCR4/CCR7 on CLL cells. Increased CCR4/CCR7 is detectable not only in circulating leukemic cells, but also in secondary lymphoid organs of CLL patients with lymphoadenopathy. PMID: 26282174
  43. in given donor/recipient pairs, KIR2DS4 expression may contribute to potentiating natural killer cell function by increasing both the cytotoxicity and the expression of CCR7 on their surface. PMID: 25961063
  44. Data indicate that inhibition of TGF-beta-activated protein kinase 1 (TAK1) reduces chemokine (C-C motif) receptor 7 (CCR7) expression. PMID: 25557171
  45. CCL21/CCR7 pathway activates signallings to up-regulate Slug pathway, leading to the occurrence of epithelial-mesenchymal transition process in human chondrosarcoma PMID: 25556164
  46. our study suggested that CCR7 promotes Snail expression to induce the epithelial-mesenchymal transition, resulting in cell cycle progression, migration, and invasion in gastric cancer PMID: 25572817
  47. High CCR7 expression was associated with colorectal cancer metastasis. PMID: 26179425
  48. The solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. PMID: 26115234
  49. These results reveal that CCR7 and VEGF-C display a significant crosstalk and suggest a novel role of the CCL21/CCR7 chemokine axis in the promotion of breast cancer-induced lymphangiogenesis. PMID: 25744065
  50. High expression of CCR-7 is associated with atherosclerotic arteries. PMID: 25318003
  51. CCR7 via RhoA/ROCK-Pyk2 cofilin pathway promotes invasion and migration of metastatic SCCHN cells PMID: 25434638
  52. JAk2/STAT3 plays a key role in CCR7 regulatingSquamous cell carcinoma of the head and neck metastasis. PMID: 25405202
  53. Data show the effect of C-terminus mutation of CC chemokine receptor 7 (CCR7) on lymphocyte migration. PMID: 25733353
  54. Therefore, our study demonstrates that MAPK members (ERK1/2 and JNK) play a key role in CCR7 regulating SCCHN metastasis. PMID: 25270024
  55. CCR7 can also promote survival of mDCs through a novel MEK1/2-ERK1/2-AMPK signaling axis. PMID: 25425646
  56. Suggest that CCRL1 impairs chemotactic events associated with CCR7 in the progression and metastasis of hepatocellular carcinoma. PMID: 25255875
  57. Downmodulation of CCR7 did not involve degradation or endocytosis and was strictly dependent on Vpu expression. PMID: 24910430
  58. Studied expression of CCR7 and EMT markers in the primary breast carcinoma tissues from patients who underwent radical mastectomy. and investigated whether CCL21/CCR7 induces EMT process during mediating cancer cell invasion or migration in vitro. PMID: 25142946
  59. Our findings show that the molecular cues secreted by TALs alone or in combination with CCR7 may emerge as future therapeutic targets for lymph node metastasis in breast cancer patients. PMID: 25327843
  60. CCR7 can constitutively express in epithelial ovarian carcinomas and be induced rapidly in response to hypoxia, which indeed participates in EMT development and prompts the cell migration and invasion. PMID: 25168373
  61. These results demonstrated the significant clinicopathological relationship and functional causality between CCR7 expression and lymph node metastasis in ESCC patients. PMID: 24766770
  62. Data indicated that CCR7 regulates cell chemotaxis and migration via MMP-9 in metastatic squamous cell carcinoma of head and neck. PMID: 24912620
  63. Except for an increase in the number of C-C chemokine receptor 7-expressing DC from MS patients, no major differences were found between groups in the expression of maturation-associated membrane markers PMID: 24856897
  64. CCR7 is more likely to be expressed in mycosis fungoides skin lesions with subcutaneous involvement. Activation of CCR7 promotes migration of MyLa cells (MF cell line) through the mTOR pathway. PMID: 24411580
  65. The involvement of dendritic cells and their expression of CCR7 in corneal and ocular surface diseases such as in ocular allergy, dry eye disease, immune rejection and more, are also reviewed here. PMID: 24725321
  66. Monocyte adhesion to HUVEC cells was induced by CCR7. CCR7 rs588019 was associated with coronary artery disease in a Chinese Han population. PMID: 24990231
  67. The TRIMEL induces a phenotypic maturation and increases the expression of surface CCR7 on melanoma patient-derived DCs, and also on the monocytic/macrophage cell line THP-1. PMID: 24673602
  68. results indicate a regulatory influence of MR signaling on human T-cell migration and suggest a role for endogenous aldosterone in the redistribution of T-cell subsets to lymph nodes, involving CD62L, CCR7, and CXCR4. PMID: 24595810
  69. CCR7 ligands CCL19 and CCL21 contribute to the accumulation of dendritic cells and alveolar macrophages in the inflamed lungs of patients with eosinophilic pneumonia. PMID: 24111618
  70. High CCR7 expression in gastric cancer cells was significantly associated with poor overall survival and lymph node metastasis. PMID: 24040244
  71. Transwell assays revealed that VEGF-C receptor, VEGFR-3, as well as chemokine CCL21 receptor, CC chemokine receptor 7 (CCR7), were responsible for the migration of PC3 cells toward hypoxia preconditioned MSCs PMID: 23939705
  72. This is the first report demonstrating the dissociation of CCR7 and MMP-9 expression in phenotypically mature CD83(+) dendritic cells by cervical cancer cells PMID: 24136650
  73. In human histological sections of chronic thrombosed femoral veins, CCR7(+) cells were present in the fibrotic areas. PMID: 24311382
  74. data suggest that CCR7 plays a role in uveal melanoma metastasis and is associated with poor survival. PMID: 24052640
  75. In the cord blood of children with respiratory syncytial virus disease there was downregulation of interleukin 7 receptor and chemokine receptor 7, and in the severe disease subcategory, downregulation of Toll-like receptor 4. PMID: 23888080
  76. High CCR7 mRNA expressions indicated better prognoses than those of the groups with low CCR7 mRNA expressions. PMID: 23922113
  77. beta-arrestin 2 and CCR7 and PI3K phosphorylation have a role in bisoprolol reversed epinephrine-mediated inhibition of cell migration, in dendritic cells loaded with cholesterol PMID: 23290307
  78. Results suggest that the CCL21/CCR7 signaling pathway is involved in renal fibrosis in kidney transplant patients. PMID: 23498789
  79. ChemR23 forms homomers, and provide data suggesting that ChemR23 also forms heteromers with the chemokine receptors CCR7 and CXCR4. PMID: 23469143
  80. Human eosinophils express CCR7 and have multipotent responses to the known ligands of CCR7. PMID: 23449735
  81. Overexpression of CCR7 protein is associated with lymph node metastasis of gastric carcinoma. PMID: 23519840
  82. CCR7 seems to influence distinct immunological events during inflammatory responses in the central nervous system, including immune-cell entry and migration, and neuroglial interactions. PMID: 22533989
  83. A combination of Leishmania-reactive CCR7- T(effector memory) cells and Leishmania-reactive CCR7+ T(central memory) cells are identified in patients with a history of cutaneous leishmaniasis which may play a protective role against Leishmania infection. PMID: 22990666
  84. The lack of CCR7 ubiquitylation profoundly impairs immune cell migration. PMID: 22797918
  85. data suggest that CCR7 via Pyk2 and cofilin regulates the chemotaxis and migration ability of metastatic squamous cell carcinoma of the head and neck cells PMID: 22923218
  86. a rise of CD56(bright) NK cells in HIV-infected individuals, which lack CCR7-expression and strongly correlate with HIV viral load PMID: 23028633
  87. RhoA/ROCK pathway is essential for PGE2-mediated CCR7-dependent monocyte migration. PMID: 22659045
  88. let-7a suppressed breast cancer cell migration and invasion by downregulating CCR7 expression. PMID: 22251626
  89. The results demonstrated that COX-2 up-regulates CCR7 expression via AKT-mediated phosphorylation and activation of Sp1 and that this pathway is highly activated in metastatic breast cancer. PMID: 22718198
  90. In a series of experiments, the yield of cultured CD8+CCR7+ T cells by the end of a 6-day induction period varies widely; loss of CD8+CCR7+ regulatory T (Treg) cells in the elderly may be of relevance in the aging immune system. PMID: 22821963
  91. oxLDL induces an in vitro downregulation of CCR7 and CCL21, which may play a role in the reduction of dendritic cell migration from the plaques PMID: 22619482
  92. Plasmacytoid dendritic cells (pDCs) constitutively migrate into the splenic white pulp in a manner dependent on chemokine receptors CCR7 and CXCR4. PMID: 22634622
  93. Moderate-to-strong immunohistochemical CCR7 expression, found in 58 of 121 well-characterized human PDACs, correlated with high rates of lymph vessel invasion PMID: 22020953
  94. A(2A) adenosine receptor activation not only modulates CCR7 expression in both normal and inflammatory environments but also regulates macrophage migration to CCR7-specific chemoattractants. PMID: 21739164
  95. CCL21/CCR7 prevents apoptosis by upregulating the expression of bcl-2 and by downregulating the expression of bax and caspase-3 potentially via the ERK pathway in non-small cell lung cancer cell lines PMID: 22438908
  96. the use of trogocytosis as a tool to transiently express the chemokine receptor CCR7 on expanded human NK cells with the aim to enhance their homing to lymph nodes. PMID: 22498742
  97. CCR7 overexpression correlated with expression of metallothionein, while CCR10 was associated with cerebral metastases. CCR7 and CCR10 overexpressions were associated with a worse outcome independent of Breslow's tumor thickness and Clark level. PMID: 22350183
  98. The autoantigen DNA topoisomerase I interacts with chemokine receptor 7 and exerts cytokine-like effects on dermal fibroblasts. PMID: 21953548
  99. the first report to link the two signaling events that control migration through the lymph nodes: CCR7 mediates entry into the lymph nodes and EDG-1 signaling controls their subsequent exit. PMID: 22334704
  100. an important biological role for inflammatory NF-kappaB and AP1 in the regulation of CCR7 expression in metastatic SCCHN. PMID: 22158872

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
Database Links

HGNC: 1608

OMIM: 600242

KEGG: hsa:1236

STRING: 9606.ENSP00000246657

UniGene: Hs.370036

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