Human C-C chemokine receptor type 6(CCR6) ELISA kit

Code CSB-EL004845HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P51684
Lead Time 7-14 working days
Abbreviation CCR6
Target Name chemokine (C-C motif) receptor 6
Alias BN-1, CD196, CKR-L3, CKR6, CKRL3, CMKBR6, DCR2, DRY-6, GPR-CY4, GPR29, GPRCY4, STRL22, G protein-coupled receptor 29|OTTHUMP00000017618|chemokine (C-C) receptor 6|chemokine receptor-like 3|seven-tra
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenetes
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.
HGNC 1607
RGD 1308562
MGI 1333797
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCR6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %90-98
1:2Average %103
Range %98-106
1:4Average %87
Range %83-91
1:8Average %98
Range %92-101
Recovery
The recovery of human CCR6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9388-96
EDTA plasma (n=4)10197-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.574 2.491 2.533 2.400
1001.637 1.596 1.617 1.484
501.108 1.098 1.103 0.970
250.672 0.653 0.663 0.530
12.50.451 0.444 0.448 0.315
6.250.287 0.274 0.281 0.148
3.120.212 0.216 0.214 0.081
00.134 0.132 0.133
References
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Function Receptor for the C-C type chemokine CCL20
Subcellular Location Cell membrane, Multi-pass membrane protein, Cell surface
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein
Database Links

HGNC: 1607

OMIM: 601835

KEGG: hsa:1235

STRING: 9606.ENSP00000339393

UniGene: Hs.46468

Pathway Chemokine signaling pathway

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