Human C-C motif chemokine 3-like 1(CCL3L1) ELISA kit

Instructions
Code CSB-EL004794HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-C motif) ligand 3-like 1
Alternative Names 464.2 ELISA Kit; C-C motif chemokine 3-like 1 ELISA Kit; CCL3L3 ELISA Kit; CL3L1_HUMAN ELISA Kit; D17S1718 ELISA Kit; G0/G1 switch regulatory protein 19-2 ELISA Kit; G0S19-2 ELISA Kit; LD78 ELISA Kit; LD78-beta(1-70) ELISA Kit; LD78-beta(5-70) ELISA Kit; LD78BETA ELISA Kit; PAT 464.2 ELISA Kit; SCYA3L ELISA Kit; SCYA3L1 ELISA Kit; Small-inducible cytokine A3-like 1 ELISA Kit; Tonsillar lymphocyte LD78 beta protein ELISA Kit
Abbreviation CCL3L1
Uniprot No. P16619
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCL3L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-94
1:2 Average % 107
Range % 98-111
1:4 Average % 94
Range % 87-98
1:8 Average % 91
Range % 84-95
Recovery
The recovery of human CCL3L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 106 98-109
EDTA plasma (n=4) 101 95-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.096 2.000 2.048 1.943
750 1.757 1.676 1.717 1.612
375 1.198 1.153 1.176 1.071
187.5 0.802 0.834 0.818 0.713
94 0.513 0.500 0.507 0.402
47 0.332 0.342 0.337 0.232
23.5 0.235 0.227 0.231 0.126
0 0.104 0.106 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1-infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5.
Gene References into Functions
  1. severe periodontal tissue destruction in Papillon-Lefevre syndrome may be related to excess accumulation of LD78beta and LD78alpha and dysregulation of the microbial-induced inflammatory response in the periodontium PMID: 15728180
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

HGNC: 10628

OMIM: 601395

KEGG: hsa:414062

STRING: 9606.ENSP00000408984

UniGene: Hs.454713

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