Human C3a anaphylatoxin chemotactic receptor(C3AR1) ELISA kit

Instructions
Code CSB-EL003889HU
Size 96T,5×96T,10×96T
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Product Details

Target Name complement component 3a receptor 1
Alternative Names AZ 3B ELISA Kit; AZ3B ELISA Kit; C3a anaphylatoxin chemotactic receptor ELISA Kit; C3A R ELISA Kit; C3A R1 ELISA Kit; C3a Receptor ELISA Kit; C3a-R ELISA Kit; C3AR ELISA Kit; C3AR_HUMAN ELISA Kit; C3AR1 ELISA Kit; C3R1 ELISA Kit; Complement component 3 receptor 1 ELISA Kit; Complement component 3a receptor 1 ELISA Kit; HNFAG09 ELISA Kit
Abbreviation C3AR1
Uniprot No. Q16581
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C3AR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 108
Range % 100-116
1:2 Average % 103
Range % 101-107
1:4 Average % 88
Range % 84-93
1:8 Average % 95
Range % 90-98
Recovery
The recovery of human C3AR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 90-102
EDTA plasma (n=4) 89 85-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.072 2.141 2.107 1.946
800 1.498 1.602 1.550 1.389
400 0.879 0.887 0.883 0.722
200 0.551 0.564 0.558 0.397
100 0.390 0.403 0.397 0.236
50 0.303 0.301 0.302 0.141
25 0.236 0.241 0.239 0.078
0 0.167 0.155 0.161  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Gene References into Functions
  1. High C3aR1 expression is associated with acute myeloid leukemia-M2. PMID: 25426664
  2. these findings suggest a critical role for C3a through autocrine/paracrine induction of C3aR in the pathogenesis of cigarette smoke-induced sterile inflammation and provide new therapeutic targets for the treatment of emphysema. PMID: 25465103
  3. Medulloblastoma cells express C3aR, and siRNA-mediated knockdown of C3aR inhibits proliferation of these cells in vitro. PMID: 25603857
  4. Observed a positive correlation between the expression of anaphylatoxin-receptors C3aR and C5aR with platelet activation in patients with coronary artery disease. PMID: 25544179
  5. C3aR and CD46 activation via intrinsic generation of their respective ligands is an integral part of human Th1 (but not Th2) immunity. PMID: 24321396
  6. functional regulation of C3aR by NHERFs in human mast cells PMID: 23284683
  7. significantly decreased expression of C3aR mRNA and protein expression in placentas with preeclampsia compared to controls PMID: 22901903
  8. study demonstrates that although C3a causes phosphorylation of its receptor at multiple sites, Ser459, Thr463, Ser465, Thr466 and Ser470 participate in C3aR desensitization PMID: 23077507
  9. Complement C3a receptor activation contributes to the pathogenesis of preeclampsia. PMID: 22868393
  10. a new level of complexity for C3aR regulation PMID: 21799898
  11. shows that both beta-arrestin-1 and beta-arrestin-2 play a novel and shared role in inhibiting G protein-dependent ERK1/2 phosphorylation. These findings reveal a new level of complexity for C3aR regulation by beta-arrestins in human mast cells PMID: 21589858
  12. Mutant gonococci lacking the pilin glycan did not bind to the I-domain when it is in a closed, low-affinity conformation and cannot induce an active conformation to complement receptor 3 during pex cell challenge. PMID: 21371235
  13. Sulfation of tyrosine 174 in the human C3a receptor is essential for binding of C3a anaphylatoxin. PMID: 12871936
  14. determination of whether the levels of complement factors C3a, C4a, and C5a are elevated at the site of inflammation in chronic obstructive pulmonary disease and in asthma PMID: 15039137
  15. Complement receptor 3 (CR3) is involved in the binding of Leishmania promastigote surface antigen-2 leucine-rich repeat motifs to macrophages, an interaction which can be blocked by anti-CR3 antibodies. PMID: 15067069
  16. C3a receptor is highly expressed in primary renal proximal tubular epithelial cells in studies that define a new pathway by which complement activation may directly modulate the renal response to immunologic injury. PMID: 15356170
  17. C3aR is expressed on plasmacytoid dendritic cells. PMID: 16778800
  18. endothelial cells and subendothelial smooth muscle cells express both C3aR and C5aR PMID: 17234193
  19. C3a receptor may be used as a unique biomarker of diagnosis and disease activity in patients with lupus nephritis. PMID: 17472841
  20. In stenotic valves, expression of C3aR mRNA was upregulated PMID: 17498719
  21. on the basis of the location of C3aR and C5aR, C5aR may play a role in activation of inflammatory cells, whereas C3aR may mediate mucus secretion and mucosal swelling in allergic nasal mucosa, especially severe persistent allergic nasal mucosa PMID: 18538384

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.
Database Links

HGNC: 1319

OMIM: 605246

KEGG: hsa:719

STRING: 9606.ENSP00000302079

UniGene: Hs.504656

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