Human CD27 antigen(CD27) ELISA kit

Instructions
Code CSB-EL004910HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name CD27 molecule
Alternative Names CD 27 ELISA Kit; CD27 ELISA Kit; CD27 antigen ELISA Kit; CD27 molecule ELISA Kit; CD27_HUMAN ELISA Kit; CD27L receptor ELISA Kit; LPFS2 ELISA Kit; MGC20393 ELISA Kit; OTTHUMP00000238557 ELISA Kit; S152 ELISA Kit; T cell activation antigen CD27 ELISA Kit; T cell antivation antigen S152 ELISA Kit; T-cell activation antigen CD27 ELISA Kit; T14 ELISA Kit; TNFRSF 7 ELISA Kit; TNFRSF7 ELISA Kit; TNFSF7 ELISA Kit; Tp 55 ELISA Kit; Tp55 ELISA Kit; Tumor necrosis factor receptor superfamily member 7 ELISA Kit
Abbreviation CD27
Uniprot No. P26842
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.81 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 93
Range % 88-98
1:10 Average % 103
Range % 100-106
1:20 Average % 92
Range % 88-97
1:40 Average % 94
Range % 91-97
Recovery
The recovery of human CD27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 93-104
EDTA plasma (n=4) 95 92-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
500 2.457 2.611 2.534 2.360
250 2.041 2.131 2.086 1.912
125 1.422 1.454 1.438 1.264
62.5 0.896 0.921 0.909 0.735
31.25 0.528 0.533 0.531 0.357
15.62 0.344 0.354 0.349 0.175
7.81 0.263 0.266 0.265 0.091
0 0.170 0.178 0.174  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Gene References into Functions
  1. Results suggests that CD27 could be a marker for poor prognosis for non-ETV6-RUNX1 B-cell precursor acute lymphoblastic leukemia. PMID: 28649984
  2. The authors observed differential expression of CD95(Fas) in CD27(+) B-cells from cirrhotic patients that was inversely correlated with peripheral CD27(+) B-cell frequency. They conclude that peripheral CD27(+) memory B-cells in cirrhosis exhibit increased sensitivity to Fas-induced apoptosis in an activation-dependent manner to which endotoxin contributes, associated with reduced frequency of circulating memory B-cells. PMID: 27857173
  3. Preclinical testing supports the safety and efficacy of a CD27-containing CAR targeting CD70-expressing tumors PMID: 27803044
  4. Crystal structure of CD27 in complex with a neutralizing noncompeting antibody has been reported. PMID: 28471362
  5. High CD27 expression is associated with B-cell Lymphoma. PMID: 28108506
  6. activated CD70-expressing lymphocytes may trigger CD27 on AML blasts or stem/progenitor cells PMID: 28031480
  7. the CD70-CD27 pathway appears to be a crucial component of EBV-specific T cell immunity and more generally for the immune surveillance of B cells and may be a target for immunotherapy of B cell malignancies. PMID: 28011863
  8. This study demonstrates increased level of sCD27 in patients with active vitiligo. PMID: 27556155
  9. This study demonstrated that Soluble CD27 in cerebrospinal fluid of patients with Clinically Isolated Syndrome was associated with multiple sclerosis diagnosis and a high relapse rate. PMID: 28055081
  10. These findings support a potential new role for B cells in dengue pathogenesis, and sCD27 and sCD38 are novel biomarkers associated with clinical outcome during dengue virus infection. PMID: 27467579
  11. Novel mutations in TNFRSF7/CD27 cause EBV-associated lymphoproliferative disease/hemophagocytic lymphohistiocytosis, Hodgkin lymphoma, uveitis, and recurrent infections. PMID: 25843314
  12. NFKB1, CD27, LAG3 and IKZF3 are new susceptibility genes for psoriasis. PMID: 25006012
  13. results provide the first evidence for differential expression of CD27 among CLL prognostic groups, suggest a role for ZAP-70 dependent signaling in CD27 induction and implicate CD27 in cell-cell interactions with the lymphoid tissue microenvironment PMID: 26002513
  14. Multiple layers of memory B cells to T cell- dependent antigens describe immunoglobulin IgM+ B cells representing a memory reservoir that can re-enter the germinal center, ensuring replenishment of class-switched memory CD27+ B cells. PMID: 24965774
  15. Data indicate a unique expansion of CD27(high) plasmablasts in both the cerebrospinal fluid and periphery of transverse myelitis (TM). patients PMID: 23594958
  16. CD27positive B cells from common variable immunodeficiency (CVID) patients are more sensitive than CD27negative B cells to spontaneous apoptosis and less sensitive to rescue from apoptosis; these B cells fail to differentiate to memory or plasma cells. PMID: 23738704
  17. lack of functional CD27 predisposes towards a combined immunodeficiency associated with potentially fatal EBV-driven hemo-phagocytosis, lymphoproliferation, and lymphoma development. PMID: 22801960
  18. The CCR7+CD45RA-CD27+CD28+ central memory subset is significantly decreased in the peripheral blood CD4+ T cells from rheumatoid arthritis patients. PMID: 23747721
  19. We identified soluble CD27 as an immune modulator associated with enhanced human T cell activation PMID: 23677477
  20. These results highlight the importance of the CD27-CD70 costimulation pathway for the development of CMV-specific T cell immunity during acute and persistent infection. PMID: 23576505
  21. The expression of CD27 together with CD44 showed a relationship with several established risk factors as well as response to therapy, indicating the biological significance of these molecules in acute lymphoblastic leukemia PMID: 23263849
  22. CD27 and CD28 costimulatory receptors play central roles in differentiation and activation of gamma-delta T Lymphocytes. (Review) PMID: 23456842
  23. IgM(+)IgD(+)CD27(+) but not switched B cells were strongly reduced in MYD88-, IRAK-4-, and TIRAP-deficient patients, but not UNC-93B-deficient patients. PMID: 23002119
  24. serum sCD27 level is associated with CD27 expression on lymphoma cells, and may have a role in diffuse large B-cell lymphoma PMID: 22280534
  25. These results indicate that CD27 and CD70 gene polymorphisms may affect the risk of breast cancer and show that some SNPs are associated with breast cancer characteristics in a northern Chinese population. PMID: 22399187
  26. Decreased CD27 on B lymphocytes in patients with primary hepatocellular carcinoma. PMID: 22429370
  27. gene deficiency is associated with combined immunodeficiency and symptomatic EBV viremia PMID: 22197273
  28. down-regulation of CD27 on MTB-specific CD4 T cell could be used as a biomarker of active TB, potentially preceding clinical TB disease PMID: 22087280
  29. Data show that in a mouse model of chronic myelogenous leukemia, BCR/ABL+ leukemia stem and progenitor cells express CD27, and reveal a mechanism by which adaptive immunity contributes to leukemia progression. PMID: 22232214
  30. Data show that CD27(+)/IgD(-)/ZAP70(+) memory B cells accumulate preferentially in the joints of RA, suggesting a dynamic maturation of the B cells in this compartment. PMID: 21607290
  31. CD27 signalling does influence T(H) cell differentiation. PMID: 21277898
  32. CD27(+) lymphocytes represent abnormal mononuclear cell populations in atrophic oral lichen planus (OLP) lesions indicating forms of OLP might exist with different pathogenesis, despite similar histology and clinical behavior. PMID: 21237436
  33. no correlation between expression on T-lymphocytes and sera levels in children with asthma PMID: 20800938
  34. CD27 may have a role in infection in systemic lupus erythematosus PMID: 20879061
  35. In this review, CD27 and CD70 constitute a unique ligand and receptor pair which can activate innate and adaptive immunity as well as regulate immunity versus tolerance. PMID: 20699361
  36. These data collectively establish a novel role for the CD70-CD27 axis in human gammadelta T-cell activation and hence open new perspectives for its modulation in clinical settings. PMID: 21182090
  37. Compared with tuberculin-positive controls, patients with bacterial culture-positive pulmonary TB had significantly reduced CD27 expression on antigen-specific CD4 T cells PMID: 20393787
  38. higher proportion of protein-expressing B-cells in the blood of patients with systemic lupus erythematosus PMID: 19866343
  39. the CD70-CD27 interaction may play an important role in inducing effective immune responses in dendritic cell-based PMID: 20201989
  40. Systemic lupus erythematosus flares may relate to the retention of CD27+ memory B cells. (review) PMID: 15230280
  41. Functionally differentiated Vgamma9delta2+ T cells lack CD27 expression and are found in reduced numbers in immunocompromised hosts (such as healthy newborns and HIV-infected adults) and during active pulmonary tuberculosis. PMID: 11801693
  42. Increased levels of soluble CD27 in the cerebrospinal fluid are not diagnostic for leptomeningeal involvement by lymphoid malignancies.The positive predictive value of sCD27 determination for LI was only 54%, but the negative predictive value was 92%. PMID: 11976819
  43. intragraft gene expression is a risk factor for acute cardiac allograft rejection PMID: 12009595
  44. IL-10 enhances B-cell IgE synthesis by promoting differentiation into plasma cells, a process that is inhibited by CD27/CD70 interaction PMID: 12197885
  45. CD27 and CD40 co-stimulatory signals regulated the p53-amplified apoptotic pathway in B cells through the inhibition of p53-independent apoptotic pathway primarily induced by BCR ligation PMID: 12324477
  46. CD27(-) effector CD8(+) T cells might be required for adequate control of chronic viral infection and prevention of disease development. PMID: 12464570
  47. CD148 and CD27 are expressed in a wide range of B cell non-Hodgkin's lymphomas and do not serve to distinguish between neoplastic cells of naive and memory B cell derivation PMID: 12685844
  48. In indolent lymphomas, sCD27 proved to be a powerful marker to predict progression-free survival (p = 0.008). PMID: 12743427
  49. Signals through CD70 in B lymphocytes of CD70 transgenic mice result in enhanced cell cycle entry while preventing differentiation into IgG-secreting plasma cells. PMID: 15356138
  50. In primary biliary cirrhosis CpG led to a markedly high frequency of intracellular IgM-positive B cells, associated with high levels of synthesized IgM and identified to be a function of CD27(+) memory B cells. PMID: 15685542

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Involvement in disease Lymphoproliferative syndrome 2 (LPFS2)
Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Found in most T-lymphocytes.
Database Links

HGNC: 11922

OMIM: 186711

KEGG: hsa:939

STRING: 9606.ENSP00000266557

UniGene: Hs.355307

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