Human CD27 antigen(CD27) ELISA kit

Code CSB-EL004910HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P26842
Lead Time 5-7 working days
Abbreviation CD27
Protein Biological Process 1 Apoptosis/Autophagy
Target Name CD27 molecule
Alias MGC20393, S152, T14, TNFRSF7, Tp55, CD27 antigen|CD27L receptor|T cell activation antigen CD27|T cell antivation antigen S152|tumor necrosis factor receptor superfamily, member 7
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, tissue homogenates
Detection Range 7.81 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
HGNC 17712
RGD 1561872
MGI 1353606
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 93
Range % 88-98
1:10 Average % 103
Range % 100-106
1:20 Average % 92
Range % 88-97
1:40 Average % 94
Range % 91-97
Recovery
The recovery of human CD27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 93-104
EDTA plasma (n=4) 95 92-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
500 2.457 2.611 2.534 2.360
250 2.041 2.131 2.086 1.912
125 1.422 1.454 1.438 1.264
62.5 0.896 0.921 0.909 0.735
31.25 0.528 0.533 0.531 0.357
15.62 0.344 0.354 0.349 0.175
7.81 0.263 0.266 0.265 0.091
0 0.170 0.178 0.174  
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Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Involvement in disease Lymphoproliferative syndrome 2 (LPFS2)
Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Found in most T-lymphocytes.
Database Links

HGNC: 11922

OMIM: 186711

KEGG: hsa:939

STRING: 9606.ENSP00000266557

UniGene: Hs.355307

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