Human CD48 antigen(CD48) ELISA kit

Instructions
Code CSB-EL004941HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name CD48 molecule
Alternative Names Antigen CD48 ELISA Kit; B cell membrane protein ELISA Kit; B lymphocyte activation marker BLAST 1 ELISA Kit; B-cell activation marker ELISA Kit; B-lymphocyte activation marker BLAST-1 ELISA Kit; BCM 1 surface antigen ELISA Kit; BCM1 ELISA Kit; BCM1 surface antigen ELISA Kit; BLAST 1 ELISA Kit; BLAST ELISA Kit; BLAST1 ELISA Kit; CD 48 ELISA Kit; CD48 ELISA Kit; CD48 antigen (B cell membrane protein) ELISA Kit; CD48 antigen ELISA Kit; CD48 molecule ELISA Kit; CD48 protein ELISA Kit; CD48_HUMAN ELISA Kit; hCD48 ELISA Kit; Leucocyte antigen MEM 102 ELISA Kit; Leukocyte antigen MEM-102 ELISA Kit; mCD48 ELISA Kit; MEM 102 ELISA Kit; MEM-102 ELISA Kit; MEM102 ELISA Kit; Signaling lymphocytic activation molecule 2 ELISA Kit; SLAM family member 2 ELISA Kit; SLAMF 2 ELISA Kit; SLAMF2 ELISA Kit; TCT.1 ELISA Kit
Abbreviation CD48
Uniprot No. P09326
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 82-94
1:2 Average % 98
Range % 93-103
1:4 Average % 92
Range % 89-96
1:8 Average % 104
Range % 97-108
Recovery
The recovery of human CD48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 89-98
EDTA plasma (n=4) 88 82-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
400 2.864 2.935 2.900 2.759
200 2.225 2.103 2.164 2.023
100 1.356 1.301 1.329 1.188
50 0.735 0.712 0.724 0.583
25 0.412 0.433 0.423 0.282
12.5 0.277 0.287 0.282 0.141
6.25 0.222 0.227 0.225 0.084
0 0.142 0.140 0.141  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.
Gene References into Functions
  1. soluble CD48 levels were significantly elevated in patients with nonallergic asthma compared to control and to the allergic asthma cohort PMID: 30306094
  2. mCD48 and sCD48 are differentially expressed in the peripheral blood of asthma patients of varying severity. sCD48 inhibits CD244-mediated eosinophil activation. These findings suggest that CD48 may play an important role in human asthma. PMID: 27859399
  3. The present study provides further insights into the role of the 2B4-CD48 interaction in the fine regulation of CD8(+) T-cell effector function upon antigenic stimulation. PMID: 26860368
  4. Data show that 2B4 not only can bind to CD48 in trans but also interacts with CD48 in cis by using the same binding interface. Also, the results demonstrated that constitutive phosphorylation of 2B4 occurs only in the presence of CD48, and that cis binding is sufficient to induce substantial levels of baseline phosphorylation. PMID: 27249817
  5. Our data indicate sCD48 as a SEB-induced 'decoy' receptor derived from eosinophil and therefore as a potential anti-inflammatory tool in S. aureus-induced eosinophil inflammation often associated with allergy. PMID: 26836239
  6. CD48 expression was increased in patients with a short disease duration compared to both controls and patients with longer disease duration. In patients with short disease duration, increased CD48 expression was associated with alveolar inflammation. PMID: 26926492
  7. These data demonstrate the important role of CD48 in SA/exotoxins-eosinophil activating interactions that can take place during allergic responses and indicate CD48 as a novel therapeutic target for allergy and especially of AD. PMID: 25255823
  8. Blockade of 2B4/CD48 interaction resulted in improvement in function via perforin expression and degranulation as measured by CD107a surface mobilization on HTLV-1 specific CD8+ T cells. PMID: 24505299
  9. we propose that SLAMF2 engagement regulates adaptive immune responses PMID: 24670806
  10. Monocyte-induced natural killer cell dysfunction was markedly attenuated by blocking CD48 receptor 2B4 on NK cells, but not by blockade of NKG2D and NKp30. PMID: 23225218
  11. replication study of association of 2 SNPs in HERV-K18 and 19 tagSNPs in CD48 with schizophrenia (SZ)and type 2 diabetes (T2D) in patients with SZ in 2 Danish samples; no association was found with SZ or with T2D among individuals with SZ for any of the SNPs PMID: 22495247
  12. Stimulation of CD48 induces rearrangement of signaling factors in lipid rafts, Lck-kinase activity, and tyrosine phosphorylation. PMID: 20833258
  13. The ligand (CD48) of the 2B4 receptor can exert both activating and inhibiting signals; natural killer (NK) cells might be at risk for self-killing were it not for the inhibiting signals generated by the 2B4-CD48 interaction. PMID: 20164429
  14. CM1-induced apoptosis is achieved via different initiation pathways, which are cell-type dependent PMID: 12072193
  15. Signal-dependent adhesion of resting NK cells initiated by expression of ICAM-1 is greatly enhanced by coexpression of CD48, even in the absence of cytokines. PMID: 12496412
  16. Engagement of natural killer (NK) cell receptor 2B4 by its counterreceptor, CD48, expressed on target cells leads to an inhibition in NK cytotoxicity independent of signaling lymphocytic activation molecule-associated protein (SAP) expression. PMID: 15356144
  17. IL-18, IL-18 receptor alpha, and CD48 complex formation via glycosylphosphatidylinositol anchor glycan triggers binding to IL-18 receptor beta, and thereby induces intracellular signal transduction and IFN-gamma production. PMID: 15760905
  18. Review of recent studies suggests an important role for interactions between 2B4 and CD48 in the course of T cell activation and proliferation PMID: 16081768
  19. 2B4 (CD244) can stimulate NK cell cytotoxicity and cytokine production by interacting with NK cell expressed CD48 and adds CD48 to the growing number of activating NK cell receptors PMID: 16585556
  20. CD48 is an interleukin (IL)-3-induced activating receptor on eosinophils and may be involved in promoting allergic inflammation. PMID: 16785501
  21. In conclusion, we cannot confirm a role of human endogenous retrovirus-K18 superantigen polymorphisms or of the CD48 CA repeat for type 1 diabetes susceptibility. PMID: 16866884
  22. findings indicate that FimH induces host cell signalling cascades that are involved in E. coli K1 invasion of human brain microvascular endothelial cells (HBMEC) and CD48 is a putative HBMEC receptor for FimH PMID: 17222190
  23. the mechanism of signal transduction by CD244 is to regulate FYN kinase recruitment and/or activity and the outcome of CD48/CD244 interactions is determined by which other receptors are engaged. PMID: 17599905

Show More

Hide All

Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Database Links

HGNC: 1683

OMIM: 109530

KEGG: hsa:962

STRING: 9606.ENSP00000357025

UniGene: Hs.243564

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1