Name: Human Carbonyl reductase [NADPH] 1 (CBR1/CBR/CRN) ELISA kit
Brand: CUSABIO
SKU: CSB-E17009h

Product Details

Target Name

carbonyl reductase 1

Alternative Names

15 hydroxyprostaglandin dehydrogenase [NADP+] ELISA Kit; 15-hydroxyprostaglandin dehydrogenase [NADP+] ELISA Kit; Carbonyl reductase [NADPH] 1 ELISA Kit; Carbonyl Reductase 1 ELISA Kit; CBR 1 ELISA Kit; CBR1 ELISA Kit; CBR1_HUMAN ELISA Kit; CRN ELISA Kit; NADPH dependent carbonyl reductase 1 ELISA Kit; NADPH-dependent carbonyl reductase 1 ELISA Kit; Prostaglandin 9 ketoreductase ELISA Kit; Prostaglandin 9-ketoreductase ELISA Kit; Prostaglandin E(2) 9 reductase ELISA Kit; Prostaglandin-E(2) 9-reductase ELISA Kit; SDR21C1 ELISA Kit

Abbreviation

CBR1

Uniprot No.

P16152

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CBR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-97
1:2	Average %	85
	Range %	80-89
1:4	Average %	94
	Range %	90-98
1:8	Average %	102
	Range %	98-107

Recovery

The recovery of human CBR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-100
EDTA plasma (n=4)	89	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.319	2.214	2.267	2.142
1000	1.394	1.452	1.423	1.298
500	0.825	0.870	0.848	0.723
250	0.471	0.487	0.479	0.354
125	0.308	0.295	0.302	0.177
62.5	0.247	0.239	0.243	0.118
31.25	0.153	0.161	0.157	0.032
0	0.122	0.128	0.125

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human CBR1 ELISA Kit was designed for the quantitative measurement of Human CBR1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

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Customer Reviews and Q&A

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■ Q&A

is it possible to use Cat#E17009H Human Carbonyl reductase [NADPH] 1 (CBR1/CBR/CRN) ELISA kit 96 tests for mouse samples too, is it possible to test this maybe?

Thanks for your inquiry!
It is not recommended to use the kit CSB-E17009H to detect mouse samples.
Because the protein of mouse and human Carbonyl reductase CBR1 are different. Also the antibody specificity of 2 kits is different.
There exsits sample matrix difference between mouse and samples.
We advise you not use the kit of across species.

Target Background

Function
(From Uniprot)

NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.

Gene References into Functions

This study examined the impact of the functional single nucleotide polymorphism CBR1 rs9024 on the bioactivation of loxoprofen in a collection of human liver samples and tumor cell lines. PMID: 29851133
CRB1 is an efficient catalyst for the reduction of glutathionylated aldehydes derived from lipid peroxidation. PMID: 27562619
The ability of human carbonyl reductase 1 to efficiently catalyze the reduction of glutathionylated aldehydes derived from lipid peroxidation, that in the case of glutathionylated-4-hydroxyalkanals is associated to the ability to oxidize the hemiacetal hydroxyl group PMID: 28274719
Data suggest that fatty acids and acyl-CoAs bind competitively with respect to substrate binding to carbonyl reductase 1 (CBR1), an enzyme involved in first-pass drug metabolism in intestinal mucosa; inhibition of CBR1 by these products of digestion may lead to food-drug interactions. PMID: 28450226
AKR1C3 is the primary enzyme and CBR1 is a minor enzyme responsible for warfarin reduction in human liver cytosol. PMID: 27055738
These results suggest that CR1 induces apoptosis by activating the caspase pathway via binding to TNFR1. PMID: 26499922
Results demonstrate a trend toward decreased functional expression of selective hepatic reductases in ESRD livers. PMID: 26282591
Critical insights into the substrate selectivity of hCBR1 and the interaction between hCBR1 and glutathione. PMID: 26381805
Up-Regulation of Carbonyl Reductase 1 Renders Development of Doxorubicin Resistance in Human Gastrointestinal Cancers PMID: 26328486
CBR1 decreases promoted tumor proliferation and growth as well as invasion and metastasis; CBR1 has potential to become a new candidate for molecular targeting therapy. PMID: 25572536
Inhibition of CBR1 may increase the efficacy of daunorubicin in cancer tissue. PMID: 25541467
Protein products of AKR1C1, AKR1C2, AKR7A3, CYP3A4, and carbonyl reductase (CBR1) were found in tumors and those of AKR1C1, AKR7A3, and CBR1 correlated with their transcript levels. PMID: 25526449
we suggest that PEP-1-CBR1 protein may be a therapeutic agent for the treatment of ischemic injuries as well as oxidative-stress-induced cell damage and death. PMID: 24440593
The stimulatory effect of cortisol on CBR1 expression may partly explain the concurrent increases of cortisol and prostaglandin PGF2alpha in human amnion tissue prior to the onset of labor PMID: 24654784
Nrf2 is a novel transcriptional regulator of CBR1 genes in humans. PMID: 23247010
GSNO-induced covalent modification of cysteine residues affects the kinetic mechanism of CBR1. PMID: 23295225
regulation of human CBR1 expression by hsa-miR-574-5p and hsa-miR-921 depends upon rs9024 genotype status PMID: 23133646
This pilot study suggests that CBR1 RNA expression may be helpful in identifying AML patients at risk of developing resistance or toxicity to daunorubicin due to increased formation of daunorubicinol. PMID: 22562609
CBR1 regulates cancer cell invasion in endometrial adenocarcinomas by regulating the epithelial mesenchymal transition PMID: 22542806
Polymorphisms in CBR1 gene did not increase risk of cardiomyopathy after anthracycline treatment of childhood cancers. PMID: 22124095
a physiological role of CBR1, but not for CBR3, in S-nitrosoglutathione reduction and thus ultimately in regulation of NO signaling PMID: 21256830
This protein has been found differentially expressed in thalami from patients with schizophrenia. PMID: 20471030
analysis of the structural basis for substrate specificity in human monomeric carbonyl reductases CBR1 PMID: 19841672
the functional genetic determinant of CBR1 activity toward relevant physiological and pharmacological substrates PMID: 17344335
The functional characterization of the promoter of CBR1 is reported. PMID: 17569794
Carbonyl reductase-1 (CBR1), microsomal prostaglandin E synthase-1 and 2 (mPGES-1, mPGES-2), cytosolic prostaglandin E synthase (cPGES), aldoketoreductase (AKR1C1) and prostaglandin F synthase (AKR1C3) were all expressed in hair follicles. PMID: 17697149
These results suggested that hCBR3 and hCBR1 play distinct physiological roles. PMID: 18493841
Human carbonyl reductase 1 is an S-nitrosoglutathione reductase PMID: 18826943
CBR1 polymorphisms have a significant influence on the pharmacokinetics of doxorubicin in Asian breast cancer patients. PMID: 19016765
the nonsynonymous single nucleotide polymorphisms generating mutations OF CBR1 may alter bioavailability of anthracyclines in cancer patients PMID: 19204081

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Subcellular Location

Cytoplasm.

Protein Families

Short-chain dehydrogenases/reductases (SDR) family

Tissue Specificity

Expressed in kidney (at protein level).

Database Links

HGNC: 1548

OMIM: 114830

KEGG: hsa:873

STRING: 9606.ENSP00000290349

UniGene: Hs.88778

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Code	CSB-E17009h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now