Human Cholinesterase(BCHE) ELISA kit

Instructions
Code CSB-EL002604HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name butyrylcholinesterase
Alternative Names Acylcholine acylhydrolase ELISA Kit; BCHE ELISA Kit; Butyrylcholine esterase ELISA Kit; CHE1 ELISA Kit; CHE2 ELISA Kit; CHLE_HUMAN ELISA Kit; Choline esterase II ELISA Kit; Cholinesterase (serum) 2 ELISA Kit; Cholinesterase 1 ELISA Kit; Cholinesterase ELISA Kit; E1 ELISA Kit; Pseudocholinesterase ELISA Kit; Pseudocholinesterase E1 ELISA Kit
Abbreviation BCHE
Uniprot No. P06276
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.69 U/mL-300 U/mL
Sensitivity 1.17 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BCHE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 92-103
1:2 Average % 91
Range % 82-97
1:4 Average % 103
Range % 98-110
1:8 Average % 87
Range % 82-95
Recovery
The recovery of human BCHE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-97
EDTA plasma (n=4) 113 108-118
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/ml OD1 OD2 Average Corrected
300 1.762 1.841 1.802 1.677
150 1.288 1.236 1.262 1.137
75 0.852 0.842 0.847 0.722
37.5 0.534 0.566 0.550 0.425
18.75 0.366 0.387 0.377 0.252
9.38 0.292 0.302 0.297 0.172
4.69 0.222 0.232 0.227 0.102
0 0.128 0.121 0.125  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Gene References into Functions
  1. An increased frequency of the BChE K-allele in multiple sclerosis patients as compared to controls was found. PMID: 29358722
  2. This study validated preoperative serum BChE levels as an independent prognostic factor for urinary tract urothelial carcinoma after radical nephroureterectomy. PMID: 29177431
  3. we present and examine means of manipulating brain BChE levels by viral gene transfer, either regionally or globally, to modulate ghrelin signaling for long-term therapeutic purposes and to set the stage for exploring the neurophysiological impact of such an intervention. PMID: 28698452
  4. The BChE-K polymorphisms are associated with deleterious changes in cognitive decline in MCI patients treated with donepezil for 3 years. PMID: 27911294
  5. BCHE-K* positive subjects (and APOE-epsilon4) display an earlier age of onset of Alzheimer's Disease and an accelerated cognitive decline. PMID: 27567841
  6. Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base.() PMID: 28544359
  7. HuBChE sequesters OP nerve agent in the bloodstream preventing the nerve agent from reaching critical target organ systems. HuBChE was effective when used as both a pre-treatment and as a post-exposure therapy. PMID: 28225154
  8. Significance of BChE Genetic Variants to Risk of Toxicity from Cholinesterase Inhibitors (review) PMID: 27551784
  9. Age, sex or smoking status did not influence butyrylcholinesterase activity in a healthy population. PMID: 28465191
  10. Mutations in the butyrylcholinesterase gene were associated with prolonged effect of succinylcholine or mivacurium. PMID: 27031121
  11. prolonged apnea after suxamethonium came about as a result of butyrylcholinesterase deficiency or mutation or acquired conditions leading to a decrease in the plasma cholinesterase activity PMID: 26439437
  12. the molecular mechanisms by which point mutations may lead to silent BChE variant or alter catalytic activity, is reported. PMID: 27062896
  13. BChE expression might be regulated by alpha-linolenic acid in HepG2 cells. PMID: 27106529
  14. Nine out of 18 (50%) individuals with butyrylcholinesterase activity below 2000 U/L had a mutation in 5'UTR (32G/A), intron 2 (c.1518-121T/C) or exon 4 (c.1699G/A; the K variant mutation). PMID: 27109752
  15. No significant difference in BChE levels of healthy pregnant women and high-risk pregnant women who had undergone a cesarean section under general anesthesia. PMID: 26955768
  16. Association between polymorphisms of BCHE and vitiligo in a two-step genetic association study. Upon confirmation of genetic association, the effect was investigated by a genotype-phenotype correlation experiment. PMID: 26189613
  17. Low serum Butyrylcholinesterase levels are associated with recurrence in prostate cancer. PMID: 26223693
  18. BCHE-K is associated with a reduced risk for Alzheimer disease and Lewy body dementia whereas APOEvarepsilon4 is associated with more rapid cognitive decline. PMID: 26757188
  19. Low BChE activity as a predictor of mortality in acute myocardial infarction might be related to its association with poor cardiac function. PMID: 27468571
  20. rs1803274 polymorphism of the BCHE gene represents a risk factor for in-stent restenosis after percutaneous coronary intervention. PMID: 26497592
  21. Complement component C3 and butyrylcholinesterase activity are associated with neurodegeneration and clinical disability in multiple sclerosis. PMID: 25835709
  22. BChE is a strong predictor for cardiac mortality specifically in younger patients with acute coronary syndrome aged between 45 and 64 years. PMID: 25933219
  23. High resolution melting analysis for the butyrylcholinesterase atypical variant genotyping is a simple, rapid, sensitive and low cost method PMID: 25336127
  24. There is a link between systemic inflammatory markers, oxidative stress, the PON192rs662-Q allele and BuChE-non-UU-phenotype and their corresponding enzymatic activity which may be considered as a risk factor for the severity of rheumatoid arthritis. PMID: 25179377
  25. There is a significant decrease in serum ChE activity after severe trauma PMID: 25471831
  26. This study proves that the p.Ala34Val BChE variant determines the "silent" phenotype (unable to hydrolyze succinylcholine and mivacurium). PMID: 25264279
  27. Data indicate that butyrylcholinesterase (BChE) was significantly associated with overall survival (OS). PMID: 24951323
  28. analysis of the T920C mutation allele frequency in butyrylcholinesterase gene in an Indian population PMID: 25447891
  29. Plasma arylesterase activity is positively associated with plasma ChE specific activity in a nested case-control study. PMID: 24473115
  30. BChE-K carriers are protected from the pathological detriments of Alzheimer's disease that affect cortical thickness. PMID: 24479631
  31. results confirm the association of APOE with Abeta deposition and represent the largest known effect of BCHE on an AD-related phenotype. PMID: 23419831
  32. Results indicate that BuChE non-UU phenotype and PON 55M allele are significant risk factors for systemic lupus erythematosus. PMID: 24399815
  33. Our findings suggest that the AA genotype of rs1803274 is a risk factor for crack cocaine use, which is more addictive than powder cocaine use. PMID: 24312228
  34. We report the case of a 65-year-old patient who was diagnosed with butyrylcholinesterase resulting in prolonged paralysis and extended mechanical ventilation PMID: 24446003
  35. Two mutants of human BCHE can effectively eliminate (-)-cocaine, cocaethylene and norcocaine in simplified kinetic models of cocaine abuse and overdose associated with the concurrent use of cocaine and alcohol. PMID: 24870023
  36. BChE activity might be involved in the pathogenesis of preeclampsia through influence on lipid and lipoprotein metabolism and oxidative stress. PMID: 23650977
  37. The present data are still inconclusive with respect to a possible contribution of common genetic variants in BCHE to the pathogenesis of attention-deficit/hyperactivity syndrome. PMID: 24041656
  38. CocH3 (BCHE mutant) with an identical concentration with that of the endogenous BChE in human plasma can effectively eliminate both cocaine and norcocaine in a simplified kinetic model of cocaine abuse. PMID: 24125115
  39. BCHE 1914G allele showed higher frequency in the obese group. Carriers of 1914G BCHE allele showed lower mean BChE activity.BCHE 1914G allele is influencing physiological mechanisms related to obesity. PMID: 24001779
  40. Data indicate that high AChE affinity of the compounds was achieved by optimizing different substituents on the pyridazinone ring, without sacrificing the AChE/BuChE selectivity profile. PMID: 23466605
  41. Water in the active site gorge of the D70G mutant is more easily depleted than that in wild-type BChE. PMID: 23782236
  42. These findings show that BCHE can hydrolyze 2-Arachidonoylglycerol which may be evidence of a more specific role for BCHE in endocannabinoid regulation. PMID: 23689009
  43. This review examines the roles of gender and BuChE genotype in the phenotypic expression of Alzheimer disease[review] PMID: 22402324
  44. Elevated levels of BuChE observed in active multiple sclerosis (MS) lesions could be related to the decompaction of myelin characteristic of the disorder. PMID: 22778864
  45. [review] Butyrylcholinesterase has diverged both structurally and in terms of tissue and cellular expression patterns from acetylcholinesterase. PMID: 22750491
  46. aspirin is hydrolyzed in plasma by two enzymes, BChE and a new extracellular form of platelet-activating factor acetylhydrolase, PAFAH1b2. PMID: 23508960
  47. characterized the catalytic activities of wild-type BChE and the A199S/F227A/S287G/A328 W/Y332G mutant against both (+)- and (-)-cocaine at the same time under the same experimental conditions PMID: 22917637
  48. verify amplification and/or deletion in the ACHE, BCHE, EPHB4 and MME genes in 32 samples of sporadic breast cancer PMID: 23063927
  49. performed molecular dynamics simulation to probe the structural stability of Indian variant (L307P) in comparison with wild and other BChE variants (D70G, E497V, V142M) having differential esterase activity PMID: 23123771
  50. Alkylating molecules were synthesized and the crystal structures they form with soman-aged butyrylcholinesterase were solved. PMID: 22922115

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Involvement in disease Butyrylcholinesterase deficiency (BChE deficiency)
Subcellular Location Secreted
Protein Families Type-B carboxylesterase/lipase family
Tissue Specificity Detected in blood plasma (at protein level). Present in most cells except erythrocytes.
Database Links

HGNC: 983

OMIM: 177400

KEGG: hsa:590

STRING: 9606.ENSP00000264381

UniGene: Hs.420483

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