Human Cluster of differentiation 3,CD3 ELISA Kit

Instructions
Code CSB-E12706h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name Cluster of differentiation 3
Abbreviation CD3
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 104
Range % 99-108
1:2 Average % 94
Range % 86-99
1:4 Average % 95
Range % 89-99
1:8 Average % 109
Range % 100-114
Recovery
The recovery of human CD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 97-110
EDTA plasma (n=4) 100 95-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.527 2.456 2.492 2.396
10 1.878 1.792 1.835 1.739
5 1.026 1.041 1.034 0.938
2.5 0.591 0.564 0.578 0.482
1.25 0.368 0.358 0.363 0.267
0.625 0.240 0.237 0.239 0.143
0.312 0.154 0.149 0.152 0.056
0 0.094 0.098 0.096  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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