Human Coagulation factor XII(F12) ELISA kit

Instructions
Code CSB-EL007918HU
Size 96T,5×96T,10×96T

Product Details

Target Name coagulation factor XII (Hageman factor)
Alternative Names HAE3, HAEX, HAF, Hageman factor|beta-factor XIIa part 1|beta-factor XIIa part 2|coagulation factor XII|coagulation factor XIIa heavy chain|coagulation factor XIIa light chain
Abbreviation F12
Uniprot No. P00748
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Blood Coagulation
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human F12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %83
Range %80-86
1:2000Average %100
Range %97-103
1:4000Average %88
Range %84-92
1:8000Average %95
Range %90-100
Recovery
The recovery of human F12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9693-99
EDTA plasma (n=4)8885-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.592 2.573 2.583 2.441
102.318 2.309 2.314 2.172
51.737 1.746 1.742 1.600
2.51.125 1.168 1.147 1.005
1.250.679 0.698 0.689 0.547
0.6250.434 0.445 0.440 0.298
0.3120.291 0.275 0.283 0.141
00.141 0.143 0.142
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
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Target Data

Function Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Involvement in disease Factor XII deficiency (FA12D); Hereditary angioedema 3 (HAE3)
Subcellular Location Secreted
Protein Families Peptidase S1 family
Database Links

HGNC: 3530

OMIM: 234000

KEGG: hsa:2161

STRING: 9606.ENSP00000253496

UniGene: Hs.1321

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