Human DNA-binding protein inhibitor ID-2(ID2) ELISA kit

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Code CSB-EL010967HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q02363
Lead Time 7-14 working days
Abbreviation ID2
Target Name inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
Alias GIG8, ID2A, ID2H, MGC26389, bHLHb26, DNA-binding protein inhibitor ID2|OTTHUMP00000140258|OTTHUMP00000200297|cell growth-inhibiting gene 8|helix-loop-helix protein ID2|inhibitor of DNA binding 2|inh
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.88 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.
HGNC 5361
RGD 2859
MGI 96397
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ID2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %105
Range %100-112
1:2Average %95
Range %90-100
1:4Average %89
Range %85-93
1:8Average %86
Range %81-90
Recovery
The recovery of human ID2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9388-97
EDTA plasma (n=4)9690-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.489 2.602 2.546 2.390
7502.004 2.109 2.057 1.901
3751.348 1.409 1.379 1.223
187.50.726 0.765 0.746 0.590
940.435 0.404 0.420 0.264
470.257 0.238 0.248 0.092
23.50.214 0.220 0.217 0.061
00.151 0.160 0.156
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Function Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock
Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Highly expressed in early fetal tissues, including those of the central nervous system.
Database Links

HGNC: 5361

OMIM: 600386

KEGG: hsa:3398

STRING: 9606.ENSP00000234091

UniGene: Hs.180919

Pathway Hippo signaling pathway
TGF-beta signaling pathway
Signaling pathways regulating pluripotency of stem cells

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