Human E3 ubiquitin-protein ligase MIB2(MIB2) ELISA kit

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Code CSB-EL013803HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q96AX9
Lead Time 5-7 working days
Abbreviation MIB2
Protein Biological Process 1 Signaling Pathway
Target Name mindbomb homolog 2 (Drosophila)
Alias RP11-345P4.1, FLJ20648, FLJ39787, FLJ41406, ZZANK1, ZZZ5, mindbomb homolog 2|novelzin|skeletrophin|zinc finger, ZZ type with ankyrin repeat domain 1
Species Homo sapiens (Human)
Protein Biological Process 3 Notch signaling pathway
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 30577
MGI 2679684
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MIB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %85-97
1:2Average %95
Range %86-104
1:4Average %96
Range %87-101
1:8Average %89
Range %84-92
Recovery
The recovery of human MIB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9685-103
EDTA plasma (n=4)9386-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16001.990 2.009 2.000 1.902
8001.706 1.772 1.739 1.641
4001.352 1.385 1.369 1.271
2000.957 0.942 0.950 0.852
1000.693 0.700 0.697 0.599
500.514 0.503 0.509 0.411
250.274 0.285 0.280 0.182
00.096 0.099 0.098
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Function E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.
Subcellular Location Cytoplasm, Endosome
Tissue Specificity Expressed in skeletal muscle, and to a lesser extent in heart, brain and kidney.
Database Links

HGNC: 30577

OMIM: 611141

KEGG: hsa:142678

STRING: 9606.ENSP00000426103

UniGene: Hs.135805

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