Human Electron transfer flavoprotein subunit alpha, mitochondrial(ETFA) ELISA kit

Code CSB-EL007844HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name electron-transfer-flavoprotein, alpha polypeptide
Alternative Names EMA, GA2, MADD, electron transfer flavoprotein alpha-subunit|electron transfer flavoprotein, alpha polypeptide|glutaric aciduria II
Abbreviation ETFA
Uniprot No. P13804
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ETFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 90-100
1:2 Average % 84
Range % 80-88
1:4 Average % 90
Range % 85-94
1:8 Average % 93
Range % 88-97
Recovery
The recovery of human ETFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 84-93
EDTA plasma (n=4) 103 99-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.215 2.159 2.187 2.010
750 1.721 1.698 1.710 1.533
375 1.204 1.227 1.216 1.039
187.5 0.896 0.865 0.881 0.704
93.75 0.639 0.614 0.627 0.450
47 0.452 0.433 0.443 0.266
23.5 0.340 0.357 0.349 0.172
0 0.173 0.180 0.177  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase
Involvement in disease Glutaric aciduria 2A (GA2A)
Subcellular Location Mitochondrion matrix
Protein Families ETF alpha-subunit/FixB family
Database Links

HGNC: 3481

OMIM: 231680

KEGG: hsa:2108

STRING: 9606.ENSP00000452762

UniGene: Hs.39925

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