Human Epithelial discoidin domain-containing receptor 1(DDR1) ELISA kit

Instructions
Code CSB-EL006595HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name discoidin domain receptor tyrosine kinase 1
Alternative Names CAK ELISA Kit; CD 167 ELISA Kit; CD167 ELISA Kit; CD167 antigen-like family member A ELISA Kit; CD167a ELISA Kit; Cell adhesion kinase ELISA Kit; DDR 1 ELISA Kit; DDR ELISA Kit; DDR1 ELISA Kit; DDR1_HUMAN ELISA Kit; Discoidin domain receptor ELISA Kit; Discoidin domain receptor tyrosine kinase 1 ELISA Kit; Discoidin receptor tyrosine kinase ELISA Kit; Discoidin receptor tyrosine kinase isoform a ELISA Kit; EDDR 1 ELISA Kit; EDDR1 ELISA Kit; Epithelial discoidin domain receptor 1 ELISA Kit; Epithelial discoidin domain-containing receptor 1 ELISA Kit; Epithelial specific receptor kinase ELISA Kit; HGK2 ELISA Kit; Mammarian carcinoma kinase 10 ELISA Kit; Mammary carcinoma kinase 10 ELISA Kit; MCK-10 ELISA Kit; MCK10 ELISA Kit; NEP ELISA Kit; Neuroepithelial tyrosine kinase ELISA Kit; Neurotrophic tyrosine kinase receptor type 4 ELISA Kit; NTRK 4 ELISA Kit; NTRK4 ELISA Kit; OTTHUMP00000029343 ELISA Kit; OTTHUMP00000029344 ELISA Kit; OTTHUMP00000029345 ELISA Kit; OTTHUMP00000029346 ELISA Kit; OTTHUMP00000029347 ELISA Kit; OTTHUMP00000164863 ELISA Kit; OTTHUMP00000164867 ELISA Kit; OTTHUMP00000222080 ELISA Kit; Protein-tyrosine kinase 3A ELISA Kit; Protein-tyrosine kinase RTK-6 ELISA Kit; PTK 3 ELISA Kit; PTK 3A protein tyrosine kinase 3A ELISA Kit; PTK3 ELISA Kit; PTK3A ELISA Kit; Receptor tyrosine kinase NEP ELISA Kit; RTK 6 ELISA Kit; RTK6 ELISA Kit; TRK E ELISA Kit; TRKE ELISA Kit; Tyrosine kinase DDR ELISA Kit; Tyrosine kinase receptor E ELISA Kit; Tyrosine-protein kinase CAK ELISA Kit
Abbreviation DDR1
Uniprot No. Q08345
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DDR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 96
Range % 93-99
1:10 Average % 101
Range % 98-104
1:20 Average % 86
Range % 84-90
1:40 Average % 107
Range % 103-110
Recovery
The recovery of human DDR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 83-90
EDTA plasma (n=4) 108 104-112
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.454 2.531 2.493 2.327
5 2.038 1.987 2.013 1.847
2.5 1.300 1.402 1.351 1.185
1.25 0.753 0.768 0.761 0.595
0.625 0.454 0.462 0.458 0.292
0.312 0.312 0.301 0.307 0.141
0.156 0.239 0.221 0.230 0.064
0 0.168 0.164 0.166  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
Gene References into Functions
  1. Data (including data from studies using knockout mice) suggest that DDR1 plays role in promoting mammary tumor growth; tumor-extrinsic DDR1 appears to be required for promotion of mammary tumors by interleukin-6. PMID: 29298894
  2. our study provides a novel regulatory pathway involving TM4SF1, DDR1, MMP2 and MMP9, which promotes the formation and function of invadopodia to support cell migration and invasion in pancreatic cancer. PMID: 28368050
  3. The three well-conserved seed matched sites for miR-199a/b-5p in the discoidin domain receptor 1 (DDR1) 3'-UTR were targeted, and miRNA binding to at least two sites was required for DDR1 inhibition. PMID: 29429150
  4. E2F1 knockdown decreased the expression of discoidin domain receptor 1 (DDR1) which plays a crucial role in many fundamental processes such as cell differentiation, adhesion, migration and invasion. PMID: 29039472
  5. Furthermore, the inhibition of DDR1 with 7rh showed striking efficacy in combination with chemotherapy in orthotopic xenografts and autochthonous pancreatic tumors where it significantly reduced DDR1 activation and downstream signaling, reduced primary tumor burden, and improved chemoresponse. PMID: 28864681
  6. These findings demonstrate a critical role of miR-199a-3p/DDR1 pathway in ovarian cancer development. PMID: 28743276
  7. findings demonstrate that, in human breast cancer cells, DDR1 regulates IR expression and ligand dependent biological actions. This novel functional crosstalk is likely clinically relevant PMID: 28591735
  8. These results support an activation mechanism of DDR1 whereby collagen induces lateral association of DDR1 dimers and phosphorylation between dimers. PMID: 28590245
  9. Data suggest that IGF-I/IGF-IR system triggers stimulatory actions through both GPER and DDR1 in aggressive tumors as mesothelioma and lung tumors. PMID: 27384677
  10. we further analyzed the CpG methylation levels at the DDR1 promoter in EOC cells and found that the CpG methylation levels of DDR1 promoter correlated negatively with the expression of DDR1 along the EMT spectrum. Therefore, EMT stratification could be a potential biomarker to predict patient response to DDR1-targeting drugs. PMID: 28887161
  11. This study suggested that DDR1 and DDR2 knockdown alters brain immunity and significantly reduces the level of triggering receptor expressed on myeloid cells (TREM)-2 and microglia. PMID: 28863860
  12. Isoform b of DDR1 is responsible for collagen I-induced up-regulation of N-cadherin and tyrosine 513 of DDR1b is necessary. PMID: 27605668
  13. Reduced DDR1 expression may be implicated in impaired melanocyte adhesion process involved in vitiligo pathogenesis PMID: 26091274
  14. data demonstrate that TGF-beta1 favors linear invadosome formation through the expressions of both the inducers, such as collagen and LOXL2, and the components such as DDR1 and MT1-MMP of linear invadosomes in cancer cells. Meanwhile, our data uncover a new TGF-beta1-dependent regulation of DDR1 expression. PMID: 27720259
  15. DDR1 overexpression promoted GC cell proliferation (p < 0.05), migration (p < 0.01), and invasion (p < 0.01), and accelerated the growth (p < 0.05) as well as the microvessel formation (p < 0.01) of transplantation tumor in nude mice. PMID: 27179963
  16. Our results suggest that DDR1 is both a prognostic marker for renal clear cell carcinoma and a potential functional target for therapy PMID: 27020590
  17. Study concludes that non-canonical DDR1 signaling enables breast cancer cells to exploit the ubiquitous interstitial matrix component collagen I to undergo metastatic reactivation in multiple target organs. PMID: 27368100
  18. Upregulation of DDR1 collagen receptor is associated with breast cancer. PMID: 26655502
  19. Knockdown DDR1 reversed the effects of Galpha13 knockdown on cell-cell adhesion and proteolytic invasion in three-dimensional collagen. PMID: 26589794
  20. Data suggest SCl2 (Streptococcus pyogenes) binds to DDR (DDR1, DDR2) ectodomain w/o stimulating receptor signaling; here, protein engineering was used to construct SCl-like proteins that inhibit collagen-DDR interactions and macrophage migration. PMID: 26702058
  21. DDR1 is a key modulator of RIT activity. PMID: 26719540
  22. DDR1 expression is a prognostic indicator in pancreatic ductal carcinoma. PMID: 26297342
  23. alpha5(IV), but not alpha1(IV), promotes lung cancer cell proliferation and tumor angiogenesis through non-integrin collagen receptor DDR1-mediated ERK activation. PMID: 25992553
  24. Hypoxia can increase DDR1 expression in pituitary adenoma cells, leading to improved MMP-2 and MMP-9 secretion, and promoting pituitary adenoma cell proliferation and invasion. PMID: 26286316
  25. suggest that DDR1 suppression may enhance adipose-derived stem cell chondrogenesis by enhancing the expression of chondrogenic genes and cartilaginous matrix deposition. PMID: 25673773
  26. MT1-MMP-discoidin domain receptor 1 axis regulates collagen-induced apoptosis in breast cancer cells PMID: 25774665
  27. Defective Ca(2+) binding in a conserved binding site causes incomplete N-glycan processing and endoplasmic reticulum trapping of DDR1 and DDR2 receptors. PMID: 25470979
  28. A DDR1(Low)/DDR2(High) protein profile is associated with TNBC and may identify invasive carcinomas with worse prognosis. PMID: 25667101
  29. We uncovered ten new mutations in TNK2 and DDR1 within serous and endometrioid ECs, thus providing novel insights into the mutation spectrum of each gene in EC. PMID: 25427824
  30. Silencing of DDR1 inhibited tumor cell growth and motility, and induced TGFBI expression, both in vitro and in vivo. PMID: 25369402
  31. found an inverse correlation between ZEB1 and DDR1 expression in various cancer cell lines and in human breast carcinoma tissues PMID: 25155634
  32. DDR1 depletion blocked cell invasion in a collagen gel PMID: 25422375
  33. The expression of the DDR1 protein significantly correlated with poor disease-free survival in patients with serous ovarian cancer. PMID: 21541037
  34. High DDR1 protein expression is associated with recurrence in ameloblastomas. PMID: 24723326
  35. In this review we highlight the mechanisms whereby DDRs(DDR1 and DDR2) regulate two important processes, namely inflammation and tissue fibrosis. PMID: 24361528
  36. Report aberrant methylation of DDR1 in men with nonobstructive azoospermia. PMID: 25064398
  37. Crystal structures of DDR1 reveal a DFG-out conformation (DFG, Asp-Phe-Gly) of the kinase domain that is stabilized by a salt bridge between the activation loop and alphaD helix. Differences to Abelson kinase are observed in the DDR1 P-loop's beta-hairpin. PMID: 24768818
  38. These findings indicate that the extracellular juxtamembrane region of DDR1 is exceptionally flexible and does not constrain the basal or ligand-activated state of the receptor. PMID: 24671415
  39. N-glycosylation at the highly conserved (211)NDS motif evolved to act as a negative repressor of DDR1 phosphorylation in the absence of ligand PMID: 24509848
  40. regulation of DDRs by glucose PMID: 24018687
  41. Upregulation of DDR1 induced by collagen I may contribute to the development and progression of NSCLC. PMID: 23761027
  42. The DDR1 extracellular domain plays a crucial role in receptor oligomerization which mediates high-affinity interactions with its ligand. PMID: 23810922
  43. We show that expression of the latent LMP1 in primary human germinal center B cells, the presumed progenitors of HRS cells, upregulates discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase activated by collagen. PMID: 24136166
  44. these data suggest that NEP can augment taxane-induced apoptosis through inhibition of Akt/Bad signaling PMID: 22895534
  45. the expression of the novel collagen receptor discoidin domain receptor 1 (DDR1) by human MKs at both mRNA and protein levels and provide evidence of DDR1 involvement in the regulation of MK motility on type I collagen PMID: 23530036
  46. DDR1 expression was not predictive for patient survival in human breast cancer PMID: 23307244
  47. DD1 mRNA and protein levels were higher in patients with recurrent Hepatocellular carcinoma , suggesting this gene maybe involved in tumor invasion and metastasis. PMID: 22752569
  48. a role for the collagenase of membrane-type MMPs in regulation of DDR1 cleavage and activation at the cell-matrix interface. PMID: 23519472
  49. Discoidin domain receptors promote alpha1beta1- and alpha2beta1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PMID: 23284937
  50. Discoidin domain receptors are unique receptor tyrosine kinases in collagen-mediated signaling [review] PMID: 23335507

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Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 3: Secreted, SUBCELLULAR LOCATION: Isoform 4: Cell membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Tissue Specificity Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the b
Database Links

HGNC: 2730

OMIM: 600408

KEGG: hsa:780

STRING: 9606.ENSP00000365759

UniGene: Hs.631988

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