Human Extracellular superoxide dismutase [Cu-Zn](SOD3) ELISA kit

Instructions
Code CSB-EL022399HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name superoxide dismutase 3, extracellular
Alternative Names EC SOD ELISA Kit; EC-SOD ELISA Kit; Extracellular superoxide dismutase [Cu Zn] ELISA Kit; Extracellular superoxide dismutase [Cu-Zn] ELISA Kit; Extracellular superoxide dismutase ELISA Kit; Extracellular superoxide dismutase precursor ELISA Kit; MGC20077 ELISA Kit; SOD 3 ELISA Kit; SOD3 ELISA Kit; SODE_HUMAN ELISA Kit; Superoxide Dismutase 3 ELISA Kit; Superoxide dismutase 3 extracellular ELISA Kit
Abbreviation SOD3
Uniprot No. P08294
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, saliva, urine
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %90
Range %82-96
1:2000Average %95
Range %90-102
1:4000Average %98
Range %92-105
1:8000Average %87
Range %83-93
Recovery
The recovery of human SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-96
EDTA plasma (n=4)9592-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.772 2.747 2.760 2.624
2502.349 2.379 2.364 2.228
1251.712 1.694 1.703 1.567
62.51.056 0.993 1.025 0.889
31.20.591 0.552 0.572 0.436
15.60.372 0.364 0.368 0.232
7.80.225 0.217 0.221 0.085
00.134 0.138 0.136  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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 Q&A
Q:

We have another question:
Could you please help our customer check with your technical team - are the antibodies used in these ELISA kits polyclonal antibodies or monoclonal antibodies?
Human CXC-chemokine receptor 4(CXCR4)ELISA Kit
https://www.cusabio.com/ELISA-Kit/Human-CXC-chemokine-receptor-4CXCR4%EF%BC%89ELISA-Kit-73517.html https://www.cusabio.com/ELISA-Kit/Human-CXC-chemokine-receptor-4CXCR4%EF%BC%89ELISA-Kit-73517.html

A:
Thanks for your following up on this case!Pls check the details as below:
#CSB-E12825h,Human CXC-chemokine receptor 4(CXCR4)ELISA Kit
https://www.cusabio.com/ELISA-Kit/Human-CXC-chemokine-receptor-4CXCR4%EF%BC%89ELISA-Kit-73517.html https:/www.cusabio.com/ELISA-Kit/Human-CXC-chemokine-receptor-4CXCR4%EF%BC%89ELISA-Kit-73517.html
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
If further questions,pls let me know.
Q:

I wanted to confirm that -CSB-EL022399HU this ELISA kit is for SOD3

A:
Thanks for your inquiry!
CSB-EL022399HU
This kit is for detection of SOD3. Pls refer to https://www.uniprot.org/uniprot/P08294.

Target Background

Function
(From Uniprot)
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Gene References into Functions
  1. High SOD3 expression is associated with cardiometabolic risk factors, and distal sensorimotor polyneuropathy. PMID: 29577557
  2. SOD3 might be a novel player in thyroid tumor stroma. PMID: 28216675
  3. SOD3 protects mesenchymal stem cells against the negative effects of serum deprivation via modulation of AMP-activated protein kinase/sirtulin 1, extracellular signalregulated kinase activation, and promoted Forkhead box O3a trafficking to the nucleus. PMID: 29921412
  4. Copper chaperone Atox-1 is involved in the induction of SOD3 in a monocyte cell line. PMID: 29168020
  5. the presence of terminal sialic acids in the N-glycans of EC-SOD enhanced both the secretion and furin-mediated C-terminal cleavage of EC-SOD. These results provide new insights into how the posttranslational modifications of EC-SOD control its functions. PMID: 29029079
  6. SOD3 reduced HIF prolyl hydroxylase domain protein activity, which increased hypoxia-inducible factor-2alpha (HIF-2alpha) stability and enhanced its binding to a specific vascular endothelial cadherin promoter region. PMID: 29422508
  7. EC-SOD released from activated neutrophils affects the redox conditions of the extracellular space and may offer protection against highly reactive oxygen species such as hydroxyl radicals otherwise generated as a result of respiratory burst activity of activated neutrophils. PMID: 27394172
  8. The results of the present study demonstrate that TET1 might function as one of the key molecules in SOD3 expression through its 5mC hydroxylation in A549 cells. PMID: 28351182
  9. The SOD3 enzyme plays a role in cardiovascular disease. PMID: 26901385
  10. SOD3 expression in human idiopathic pulmonary arterial hypertension is in part regulated by histone deacetylation. PMID: 27233998
  11. These data provide new insights into the functional actions of SOD3 on oxidative stress-induced cell damage. PMID: 27272114
  12. Study shows that patients with the Ala40Thr polymorphism in EC-SOD are at a higher risk of developing type 2 diabetes mellitus. PMID: 27966735
  13. Increased expression of SOD3 ameliorates H2O2-induced oxidative damage in neuroblastoma cells by inhibiting the mitochondrial pathway. PMID: 27084770
  14. Results describe the molecular cloning of both full length and truncated form of human SOD3 both expressed in Sf9 insect cells as monomers and dimer conformation, with enzymatic activity. PMID: 26912083
  15. study suggests that carriers of adiponectin gene promoter -11391G/A(AA) and EC-SOD (CG+GG) genotypes may have a high risk of nonalcoholic fatty liver disease (NAFLD), and the gene genotypes can interact with H. Pylori infection in the pathogenesis of NAFLD PMID: 27241145
  16. FXR may regulate SOD3 expression to suppress reactive oxygen species production, resulting in decreasing JNK activity. PMID: 25496033
  17. These results support the hypothesis that loss of extracellular SOD contributes to the invasive phenotype of pancreatic ductal adenocarcinoma PMID: 25634994
  18. The T-allele of rs2284659 in the promoter of SOD3 was associated with better cardiovascular outcomes in diabetic patients. PMID: 25855220
  19. SOD3 regulates the expression of multiple components of small G protein GTPase signal pathways. PMID: 25751262
  20. Expression of extracellular superoxide dismutase (EC-SOD) and expression of the prooxidant gene NADPH oxidase 4 was decreased significantly by trichostatin A. PMID: 25749103
  21. Extracellular superoxide dismutase ameliorates streptozotocin-induced rat diabetic nephropathy via inhibiting the ROS/ERK1/2 signaling. PMID: 26006040
  22. ECSOD Ala40Thr polymorphism, a significant association was observed between this polymorphism and HCC risk in non-hepatitis B virus (HBV) carriers but not in HBV carriers PMID: 25894370
  23. The combination of serum S100A9, SOD3, and MMP9 levels could achieve 92.5% sensitivity and 95% specificity to discriminate between pulmonary tuberculosis and healthy controls. PMID: 25332062
  24. Sod3 is a critical mediator of VEGF-C-induced breast cancer metastasis. PMID: 25358638
  25. the rs1799895 polymorphism in extracellular superoxide dismutase affects cardiopulmonary disease risk by altering protein distribution PMID: 25085920
  26. The treatment with E2 suppressed, whereas VC and Res prevented E2-mediated decrease in the expression levels of SOD3, NQO1, Nrf2 mRNA, and protein in MCF-10A cells PMID: 25130429
  27. No any significantly association between SOD3 rs2695232 polymorphism and seminal superoxide dismutase activity. PMID: 24658925
  28. These results demonstrate that the transcription factor HIF-1alpha and its important gene target VEGF can be modulated by the antioxidant enzyme EcSOD. PMID: 24509158
  29. Loss of SOD3 is associated with prostate cancer. PMID: 24922645
  30. Results suggest that there is no considerable influence of sequence variation in SOD3 on human longevity in Germans. PMID: 24146173
  31. the impact of Glutamate carboxypeptidase II (GCPII) haplotypes on the expression of PSMA, BNIP3, Ec-SOD, GSTP1 and RASSF1 genes were elucidated to understand the epigenetic basis of oxidative stress and prostate cancer risk. PMID: 23979608
  32. Data suggest that epigenetic silencing of EcSOD may contribute to mammary tumorigenesis and that restoring the extracellular superoxide scavenging activity could be an effective strategy for breast cancer treatment. PMID: 23318435
  33. The hEC-SOD protein was expressed in the egg white and showed antioxidant activity. PMID: 23977988
  34. The less common allele in SOD3 rs699473 was associated with an increased risk of high-grade prostate cancer (T > C: OR = 1.40, 95% CI: 1.04-1.89). PMID: 24038157
  35. Mesenchymal stem cell-derived chondrocytes, adipocytes, and osteocytes secrete an active and functional SOD3 enzyme. PMID: 22132904
  36. Overexpression of EC-SOD combined with neutrophil blockade protects transgenic mice from hyperoxia-induced lung injury. PMID: 22816678
  37. There is a positive relationship between the prolactinoma severity and serum EC-SOD. PMID: 23620962
  38. results suggest aberrations in one-carbon metabolism appear to induce altered gene expression of EC-SOD, GSTP1, and BNIP3, and thus contribute to the increased oxidative stress and increased susceptibility to coronary artery disease PMID: 23160801
  39. Antioxidant enzyme, SOD3, reverses DNA damage response and cellular transdifferentiation in aortic valve sclerosis. PMID: 23241403
  40. studies further demonstrate that SOD3, but not SOD2 and SOD1, is induced by antioxidants and is regulated through NRF2; SOD3 may thus be an important gene in defense against oxidative stress and in the prevention of estrogen-mediated breast cancer PMID: 23027624
  41. miR-21 promotes tumorigenesis and key targets of miR-21 in mediating this function were SOD3 and TNFalpha. PMID: 22836756
  42. results suggest that the expression of Extracellular-superoxide dismutase (EC-SOD)was increased by TPA administration, and it was speculated that the activation of PKC, MEK/ERK and an increase of intracellular ROS were necessary for the induction of EC-SOD in THP-1 cells PMID: 22313459
  43. There were no significant differences in the distribution of the different genotypes or allele gene frequencies in the EC-SOD genes between the patients and the controls. PMID: 21781513
  44. The SOD3 might provide an effective strategy for the treatment of HAF-mediated skin inflammation. PMID: 21957979
  45. The loss of EcSOD expression is unique among the superoxide dismutases in lung cancer and is the result of EcSOD promoter methylation and LOH, suggesting that its early loss may contribute to ECM remodeling and malignant progression. PMID: 22064654
  46. an alteration in SOD3 expression and activity could be associated to Systemic sclerosis (SSc) fibrosis. PMID: 22217996
  47. Mushroom lectin strongly prevented sodium arsenite-induced damage of SOD production pathway in hepatocytes. PMID: 21554548
  48. Extracellular superoxide dismutase cell-specific and interferon-gamma-inducible expression in pulmonary artery cells is regulated, to a major degree, by epigenetic mechanisms that include histone acetylation and DNA methylation. PMID: 21493784
  49. elevated sputum levels in smokers and in COPD patients PMID: 21621610
  50. Extracellular superoxide dismutase facilitates clearance of bacteria and limits inflammation in response to infection by promoting bacterial phagocytosis. PMID: 21641397

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Subcellular Location Secreted, extracellular space
Protein Families Cu-Zn superoxide dismutase family
Tissue Specificity Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
Database Links

HGNC: 11181

OMIM: 185490

KEGG: hsa:6649

STRING: 9606.ENSP00000371554

UniGene: Hs.2420

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