Human Fibroblast growth factor 12(FGF12) ELISA kit

Code CSB-EL008618HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P61328
Lead Time 7-14 working days
Abbreviation FGF12
Target Name fibroblast growth factor 12
Alias FGF12B, FHF1, OTTHUMP00000209265|OTTHUMP00000209266|fibroblast growth factor 12B|fibroblast growth factor FGF-12b|fibroblast growth factor homologous factor 1|myocyte-activating factor
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 37.5 pg/mL-2400 pg/mL
Sensitivity 9.4 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
HGNC 3668
RGD 620163
MGI 109183
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FGF12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 82-91
1:2 Average % 94
Range % 89-98
1:4 Average % 107
Range % 103-110
1:8 Average % 110
Range % 104-114
Recovery
The recovery of human FGF12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 91-99
EDTA plasma (n=4) 103 98-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2400 2.066 2.103 2.085 1.978
1200 1.611 1.634 1.623 1.516
600 1.075 1.102 1.089 0.982
300 0.636 0.628 0.632 0.525
150 0.404 0.431 0.418 0.311
75 0.238 0.242 0.240 0.133
37.5 0.191 0.198 0.195 0.088
0 0.106 0.108 0.107  
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Function Involved in nervous system development and function. Involved in the positive regulation of voltage-gated sodium channel activity. Promotes neuronal excitability by elevating the voltage dependence of neuronal sodium channel SCN8A fast inactivation.
Involvement in disease Epileptic encephalopathy, early infantile, 47 (EIEE47)
Subcellular Location Nucleus
Protein Families Heparin-binding growth factors family
Tissue Specificity Brain, eye and testis; highly expressed in embryonic retina, olfactory epithelium, olfactory bulb, and in a segmental pattern of the body wall; in adult olfactory bulb, less in cerebellum, deep cerebellar nuclei, cortex and multiple midbrain structures.
Database Links

HGNC: 3668

OMIM: 601513

KEGG: hsa:2257

STRING: 9606.ENSP00000413496

UniGene: Hs.390250

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