Human Fibroblast growth factor 14(FGF14) ELISA kit

Instructions
Code CSB-EL008620HU
Size 96T,5×96T,10×96T

Product Details

Target Name fibroblast growth factor 14
Alternative Names RP11-397O8.6, FHF4, MGC119129, SCA27, bA397O8.2|fibroblast growth factor homologous factor 4
Abbreviation FGF14
Uniprot No. Q92915
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FGF14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %89-97
1:2Average %101
Range %97-104
1:4Average %89
Range %84-92
1:8Average %90
Range %86-93
Recovery
The recovery of human FGF14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9286-95
EDTA plasma (n=4)10499-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.044 1.998 2.021 1.861
7501.376 1.389 1.383 1.223
3750.784 0.778 0.781 0.621
187.50.438 0.427 0.433 0.273
940.308 0.303 0.306 0.146
470.244 0.256 0.250 0.090
23.50.208 0.202 0.205 0.045
00.161 0.159 0.160
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Probably involved in nervous system development and function.
Involvement in disease Spinocerebellar ataxia 27 (SCA27)
Subcellular Location Nucleus
Protein Families Heparin-binding growth factors family
Tissue Specificity Nervous system.
Database Links

HGNC: 3671

OMIM: 601515

KEGG: hsa:2259

STRING: 9606.ENSP00000365301

UniGene: Hs.508616

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