Human Fibroblast growth factor 22(FGF22) ELISA kit

Instructions
Code CSB-EL008628HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name fibroblast growth factor 22
Alternative Names FGF 22 ELISA Kit; FGF-22 ELISA Kit; FGF22 ELISA Kit; FGF22_HUMAN ELISA Kit; Fibroblast growth factor 22 ELISA Kit
Abbreviation FGF22
Uniprot No. Q9HCT0
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FGF22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 92-100
1:2 Average % 90
Range % 86-94
1:4 Average % 86
Range % 82-90
1:8 Average % 93
Range % 89-98
Recovery
The recovery of human FGF22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 100 95-107
EDTA plasma (n=4) 88 84-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 1.847 1.945 1.896 1.763
800 1.239 1.308 1.274 1.141
400 0.794 0.812 0.803 0.670
200 0.501 0.527 0.514 0.381
100 0.328 0.339 0.334 0.201
50 0.273 0.281 0.277 0.144
25 0.202 0.194 0.198 0.065
0 0.136 0.130 0.133  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Plays a role in the fasting response, glucose homeostasis, lipolysis and lipogenesis. Can stimulate cell proliferation (in vitro). May be involved in hair development.
Gene References into Functions
  1. This study showed that serum FGF22 levels in depressive patients were negatively correlated with serum IL-1beta levels. In animal and cell experiments, enhancing the levels of FGF22 in rat hippocampus was demonstrated to alleviate the chronic unpredictable mild stress-induced depression. The increase in FGF22 was found to be associated with reduced IL-1beta expression and hippocampal apoptosis. PMID: 28948716
Subcellular Location Secreted
Protein Families Heparin-binding growth factors family
Database Links

HGNC: 3679

OMIM: 605831

KEGG: hsa:27006

STRING: 9606.ENSP00000215530

UniGene: Hs.248087

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