Human Fibronectin type III and SPRY domain-containing protein 1(FSD1) ELISA kit

Unavailable
Code CSB-EL009013HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9BTV5
Lead Time 5-7 working days
Abbreviation FSD1
Protein Biological Process 1 Cell Cycle
Target Name fibronectin type III and SPRY domain containing 1
Alias GLFND, MGC3213, MIR1, fibronectin type 3 and SPRY (spla, ryanodine) domain containing (with coiled-coil motif) 1|fibronectin type 3 and SPRY domain containing 1|microtubule-associated protein GLFND|
Species Homo sapiens (Human)
Protein Biological Process 3 Cell cycle
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a centrosome associated protein that is characterized by an N-terminal coiled-coil region downstream of B-box (BBC) domain, a central fibronectin type III domain, and a C-terminal repeats in splA and RyR (SPRY) domain. The encoded protein associates with a subset of microtubules and may be involved in the stability and organization of microtubules during cytokinesis.
HGNC 13745
RGD 1583850
MGI 1934858
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FSD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 106
Range % 100-114
1:2 Average % 102
Range % 101-105
1:4 Average % 86
Range % 84-92
1:8 Average % 93
Range % 90-96
Recovery
The recovery of human FSD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 90-101
EDTA plasma (n=4) 87 85-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.281 2.133 2.207 2.020
750 1.820 1.855 1.838 1.651
375 1.275 1.322 1.299 1.112
187.5 0.779 0.721 0.750 0.563
93.75 0.521 0.566 0.544 0.357
46.88 0.409 0.424 0.417 0.230
23.44 0.311 0.300 0.306 0.119
0 0.189 0.185 0.187  
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Function May be involved in microtubule organization and stabilization.
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Nucleus, Cytoplasm, Cleavage furrow
Tissue Specificity Highly expressed in brain tissues, including cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Lower expression in spinal chord.
Database Links

HGNC: 13745

OMIM: 609828

KEGG: hsa:79187

STRING: 9606.ENSP00000221856

UniGene: Hs.28144

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